Anti-PLC beta 1 Antibody (16) recommended for detection of PLC β1 of mouse, rat and human origin by WB, IP and IF

Anti-PLC β1 Antibody (16): sc-136040

Anti-PLC β1 Antibody (16) is rated 4.3 out of 5 by 3.
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Datasheets

    • PLC β1 Antibody (16) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 4-159 of PLC β1 of rat origin
    • recommended for detection of PLC β1 of mouse, rat and human origin by WB, IP and IF
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PLC β1 (16): sc-136040.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PLC β1 Antibody (16). This work is in progress.
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I am using PLC β1 (16): sc-136040 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Produced publishable WB data in human This PLC beta 1 monoclonal antibody was used in a publication for WB on HEK TAP-PLCβ1 cells
Date published: 2017-02-09
Rated 4 out of 5 by from Produced good immunofluorescence cytoplasmic Produced good immunofluorescence cytoplasmic staining in human intestinal smooth muscle cells. -SCBT QC
Date published: 2015-03-05
Rated 4 out of 5 by from Robust band seen in WB using rat cerebrum Robust band seen in WB using rat cerebrum tissue extract. -SCBT QC
Date published: 2015-02-28
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