Anti-PKR Antibody (B-10) is a mouse monoclonal IgG2a κ PKR antibody, cited in 126 publications, provided at 200 µg/ml
raised against amino acids 1-515 of PKR of mouse origin
Anti-PKR Antibody (B-10) is recommended for detection of PKR of mouse, rat and human origin by WB, IP, IF and IHC(P)
Anti-PKR Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKR (B-10): sc-6282.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for PKR Antibody (B-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with PKR Antibody (B-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PKR Antibody (B-10) is a high quality monoclonal PKR antibody (also designated EIF2AK2 antibody) suitable for the detection of the PKR protein of mouse, rat and human origin. PKR Antibody (B-10) is available as both the non-conjugated anti-PKR antibody form, as well as multiple conjugated forms of anti-PKR antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the a subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2a on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA mediated autophosphorylation.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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