Anti-PKM Antibody (C-11) recommended for detection of PKM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PKM Antibody (C-11): sc-365684

Anti-PKM Antibody (C-11) is rated 4.8 out of 5 by 33.
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Datasheets

    • Anti-PKM Antibody (C-11) is a mouse monoclonal IgG2a κ PKM antibody, cited in 26 publications, provided at 200 µg/ml
    • raised against amino acids 454-513 mapping near the C-terminus of PKM of human origin
    • Anti-PKM Antibody (C-11) is recommended for detection of PKM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PKM Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKM (C-11): sc-365684.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PKM Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with PKM Antibody (C-11) (see ordering information below).
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Is this antibody specific to PKM2? or both isoforms of PKM?

Asked by: zhengx
Thank you for your question. This antibody will detect both isoforms of PKM. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2018-01-16

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-365684 PE), fluorescein (sc-365684 FITC), Alexa Fluor® 488 (sc-365684 AF488) or Alexa Fluor® 647 (sc-365684 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 10
Date published: 2017-01-10
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Rated 5 out of 5 by from Good results of WB We got a single and clear band in Western blotting analysis.
Date published: 2020-10-06
Rated 5 out of 5 by from Works well in WB Single band in western blot using HeLa whole cell extract
Date published: 2019-11-06
Rated 4 out of 5 by from Good antibody I recently got a sample and tested it with whole cell lysates in concentration 1:300. Gives a strong band but also some background. However the latter is not affecting the result as the pkm is so clear.
Date published: 2019-06-06
Rated 5 out of 5 by from Good Antibody works nice results for murine myelopbalst cell line
Date published: 2018-08-03
Rated 5 out of 5 by from wonderful antibody! 1:1000 diluted for wb of rat protein,the band is stong and special!
Date published: 2018-06-12
Rated 5 out of 5 by from 好用,条带干净 好用,背景干净,推荐,很好的购物体验,品质逐渐优化,下次应该还会尝试和推荐实验室别的santa抗体。
Date published: 2018-05-07
Rated 4 out of 5 by from Good clean bands Requested as sample. Worked well with our assay and we ordered full vial
Date published: 2018-02-05
Rated 5 out of 5 by from Escelente resultado Me facilitaron una muestra de Ab y me dió muy buen resultado
Date published: 2017-11-16
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