Anti-PKM Antibody (C-11) is a mouse monoclonal IgG2a κ PKM antibody, cited in 26 publications, provided at 200 µg/ml
raised against amino acids 454-513 mapping near the C-terminus of PKM of human origin
Anti-PKM Antibody (C-11) is recommended for detection of PKM of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-PKM Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKM (C-11): sc-365684.
m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PKM Antibody (C-11) for WB and IHC(P) applications. These reagents are now offered in bundles with PKM Antibody (C-11) (see ordering information below).
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Labware ships FedEx Ground free of charge to the contiguous US
PKM Antibody (C-11) is a high quality monoclonal PKM antibody (also designated phosphoenolpyruvate phosphoryltransferase antibody, or p58 antibody) suitable for the detection of the PKM protein of mouse, rat and human origin. PKM Antibody (C-11) is available as both the non-conjugated anti-PKM antibody form, as well as multiple conjugated forms of anti-PKM antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. In mammals, four different isoenzymes exist for pyruvate kinase. Based on their tissue distribution, the isoenzymes are designated L-type (for predominant expression in the liver), R-type (for predominant expression in red blood cells), M1-type (for predominant expression in muscle, brain and heart) and M2-type (for predominant expression in fetal tissues). Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKM (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. The M2-type isoform also interacts with Oct-4 via its C-terminal domain, functioning to enhance Oct-4 transcriptional activity.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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