PITSLRE Antibody (B-9)recommended for detection of PITSLRE A and PITSLRE B of human origin and PITSLRE of mouse and rat origin by WB, IP, IF and ELISA

PITSLRE Antibody (B-9): sc-377296

PITSLRE Antibody (B-9) is rated 5.0 out of 5 by 3.
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Datasheets

    • PITSLRE Antibody (B-9) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 749-775 at the C-terminus of PITSLRE A of human origin
    • recommended for detection of PITSLRE A and PITSLRE B of human origin and PITSLRE of mouse and rat origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • blocking peptide, sc-377296 P
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PITSLRE (B-9): sc-377296.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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How is it possible that IgM kappa antibody PITSLRE Antibody (B-9): sc-377296 could be detected with m-IgGκ BP-HRP: sc-516102 that is advertised to be specific to IgG? Are those bands shown on sc-377296 product page really specific?

Asked by: CzechScience
Thank you for your question. m-IgGκ BP-HRP: sc-516102 binds to the κ-chain of the antibody. It recognizes the κ-chains of IgG, IgM, and IgA antibodies (including PITSLRE Antibody (B-9): sc-377296). The bands shown on the product page of PITSLRE Antibody (B-9): sc-377296 are specific. For antibodies with a λ-chain we offer a variety of secondary detection proteins as well. You can find them here: https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4 Please contact our technical service if you have further questions in this regard.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What application is the blocking peptide sc-377296 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from good product It works pretty well for our western blot experiment.
Date published: 2018-08-22
Rated 5 out of 5 by from Excellent immunofluorescence nuclear and Excellent immunofluorescence nuclear and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Good Western Blot data of PITSLRE expression Good Western Blot data of PITSLRE expression in A549 and WI 38 whole cell lysates. -SCBT QC
Date published: 2013-12-18
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