PIPES sodium saltA buffering agent in biochemical applications

PIPES sodium salt (CAS 10010-67-0)

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Synonym: Piperazine-N,N′-bis(2-ethanesulfonic acid) sodium salt
Application: A buffering agent in biochemical applications
CAS Number: 10010-67-0
Purity: ≥98%
Molecular Weight: 324.35
Molecular Formula: C8H17N2O6S2•Na
* Refer to Certificate of Analysis for lot specific data (including water content).
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PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.
Additional forms available: PIPES, Free Acid (sc-216099); PIPES, Sesquisodium Salt (sc-216100); PIPES dipotassium salt (sc-258019); PIPES disodium salt (sc-264078)


1. Good, N.E., et al. 1966. Biochemistry. 5: 467-477. PMID: 5942950
2. Schiff, R.I. and Gennaro, J.F. 1979. Scan Electron Microsc. 449-458. PMID: 118516
3. Yamamoto, K. and Ogawa, K. 1983. Histochemistry. 77: 339-351. PMID: 6305886
4. Blanchard, J.S. 1984. Meth. Enzymol. 104: 404-414. PMID: 6717292

Physical State :
Solubility :
Soluble in water (0.05 M), giving a clear, colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.
pH :
useful range 6.1 - 7.5
Storage :
Store at room temperature
Melting Point :
>300° C (dec.)
pK Values :
pKa: 6.8 at 25° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
PubChem CID :
Merck Index :
14: 7479
MDL Number :
EC Number :

Download SDS (MSDS)

Certificate of Analysis

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