Pinch-1 Antibody (49)recommended for detection of Pinch-1 of mouse, rat and human origin by WB and IP

Pinch-1 Antibody (49): sc-136299

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Datasheets

    • Pinch-1 Antibody (49) is a mouse monoclonal IgG2a provided at 50 µg/0.5 ml
    • raised against amino acids 120-220 of Pinch-1 of human origin
    • recommended for detection of Pinch-1 of mouse, rat and human origin by WB and IP
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I am using Pinch-1 (49): sc-136299 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jenni
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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