Anti-PI 3-kinase p55 gamma Antibody (E-9) recommended for detection of PI 3-kinase p55γ of human origin by WB, IP, IF and ELISA

Anti-PI 3-kinase p55γ Antibody (E-9): sc-376615

Anti-PI 3-kinase p55γ Antibody (E-9) is rated 5.0 out of 5 by 3.
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Datasheets

    • Anti-PI 3-kinase p55γ Antibody (E-9) is a mouse monoclonal IgG1 κ PI 3-kinase p55γ antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against amino acids 333-380 mapping within an internal region of PI 3-kinase p55γ of human origin
    • PI 3-kinase p55 gamma Antibody (E-9) is recommended for detection of PI 3-kinase p55γ of human origin by WB, IP, IF and ELISA
    • Anti-PI 3-kinase p55 gamma Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PI 3-kinase p55γ (E-9): sc-376615.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PI 3-kinase p55γ Antibody (E-9) for WB applications. These reagents are now offered in bundles with PI 3-kinase p55γ Antibody (E-9) (see ordering information below).
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Hi, does this antibody cross react with alpha or delta, or does it react only with gamma?

Asked by: Anonymous
Thank you for your question. This antibody has not been tested for its cross-reactivity with alpha or delta isoforms. However, due to the low sequence similarity of the anitbody's epitope sequence and its equivalent sequences in the different subtypes, cross-rectivity is very improbable.
Answered by: Tech Support Europe
Date published: 2020-11-03

What is the recommended starting dilution for Western blot for sc-376615: PI 3-kinase p55γ (E-9) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from WILL BUY IT AGAIN I bought it 2 weeks ago and am so happy that it works well!
Date published: 2019-12-10
Rated 5 out of 5 by from Positive results Strong positive results with PI 3-kinase p55 gamma Antibody (E-9) by Western blot
Date published: 2017-02-02
Rated 5 out of 5 by from Worked well in WB in SK Worked well in WB in SK-N-MC and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-03-27
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