Anti-PI 3-kinase p110 alpha Antibody (4F3) recommended for detection of PI 3-kinase p110α of mouse, rat and human origin by WB, IP, IF, FCM and ELISA

Anti-PI 3-kinase p110α Antibody (4F3): sc-293172

Anti-PI 3-kinase p110α Antibody (4F3) is rated 3.3 out of 5 by 6.
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Datasheets

    • Anti-PI 3-kinase p110α Antibody (4F3) is a mouse monoclonal IgG1 κ PI 3-kinase p110α antibody, cited in 23 publications, provided at 50 µg/0.5 ml
    • raised against recombinant protein fragment corresponding to PI 3-kinase p110α of human origin
    • recommended for detection of PI 3-kinase p110α of mouse, rat and human origin by WB, IP, IF, FCM and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PI 3-kinase p110α (4F3): sc-293172.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PI 3-kinase p110α Antibody (4F3). This work is in progress.
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Could you please suggest the secondary Antibody for sc-293172. Which species it was made?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-22

Is this antibody predict to recognizes rabbit?

Asked by: Ana Mesquita
Thank you for your question. This antibody has not been tested for use on any other species than mouse, rat and human samples. As we have not verified this information, we still only recommend and warranty it for mouse, rat and human samples, but it may work in other species as well. Please contact our Technical Service Department by phone, e-mail or live chat, or your local distributor, if you have any further questions.
Answered by: Tech Service
Date published: 2017-04-25

I would like to use PI 3-kinase p110α (4F3): sc-293172 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Answered by: Technical Support
Date published: 2017-04-12
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Rated 4 out of 5 by from Western blot Positive aspect: Antibody worked well and the band is really defined (1:200 diluted in PBS-T with 5% milk). Negative aspect: In the antibody datasheet is written that the molecular weight is 110 KDa but in my western blots with cellular extracts from MCF7, MDA-MB-231 and HEK293 I saw the band in ~60 KDa. I took a lot of time to understand the problem because I was cutting the membranes near 110KDa, and so my membranes were empty of bands. Only when I did the whole membrane I understood the problem.
Date published: 2017-10-27
Rated 3 out of 5 by from sad I had tride many times ,including different electrophoresis and transmembrane conditions,but the result is still unsatisfied.
Date published: 2017-09-20
Rated 2 out of 5 by from no stain I used this antibody(free sample) to test human leukocyte.no stain. Probably because of my sample.
Date published: 2017-08-23
Rated 3 out of 5 by from 没做出效果 western blotting效果不是很理想,不知道是不是因为是磷酸化的抗体,样品放的太久了蛋白降解了?目前还没重复实验。。。。
Date published: 2017-03-23
Rated 4 out of 5 by from sc-293172 存在问题:45KD和110KD均可以发出一条带,但110KD弱,不清楚发文章到底选取哪一条带。其他挺好的。
Date published: 2017-02-23
Rated 4 out of 5 by from Worked well in WB in MCF-7 . PI3K p110 alpha antibody (sc-293172) worked well in westernblot in MCF-7 .
Date published: 2016-12-16
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