Anti-PI 3-kinase p110 Antibody (D-4) recommended for detection of PI 3-kinase p110 of human origin by WB, IP, IF and ELISA

Anti-PI 3-kinase p110 Antibody (D-4): sc-8010

Anti-PI 3-kinase p110 Antibody (D-4) is rated 4.9 out of 5 by 12.
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Datasheets

    • Anti-PI 3-kinase p110 Antibody (D-4) is a mouse monoclonal IgG2a κ PI 3-kinase p110 antibody, cited in 35 publications, provided at 200 µg/ml
    • raised against amino acids 800-1039 mapping at the C-terminus of PI 3-kinase p110 of human origin
    • PI 3-kinase p110 Antibody (D-4) is recommended for detection of PI 3-kinase p110 of human origin by WB, IP, IF and ELISA
    • Anti-PI 3-kinase p110 Antibody (D-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PI 3-kinase p110 (D-4): sc-8010.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PI 3-kinase p110 Antibody (D-4) for WB applications. These reagents are now offered in bundles with PI 3-kinase p110 Antibody (D-4) (see ordering information below).
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这个p110的亚基是哪个呀

Asked by: nnFairy1212
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Does this recognise all alpha, beta and delta isoforms?

Asked by: Anatomical Science
Thank you for your question. This mouse monoclonal antibody was raised against PIK3CB, beta isoform; amino acids 800-1039 mapping at the C-terminus of PI 3-kinase p110 of human origin, protein accession number P42338. We have not evaluated the reactivity of this antibody with all isoforms.
Answered by: Technical Support
Date published: 2019-06-21

Tienen el fosforilado de proteína PI 3-kinase p110 ?

Asked by: FernandoGarzón
Gracias por tu pregunta. No ofrecemos un anticuerpo en contra de fosforilada PI 3-quinasa p110 en este momento. Thank you for your question. We do not offer a monoclonal antibody against phosphorylated PI 3-kinase p110 at this time.
Answered by: Technical Support
Date published: 2018-12-31

I probed for P110 gamma but nothing was detected. Dilution used was 1:1000 for 1 hr at room temperature.

Asked by: Srav
Thank you for your question. We recommend using this primary antibody at a starting dilution of 1:200 for Western blotting. Below is a link to our complete recommended protocol for Western blot. Please contact our Technical Service Department if you would like support in troubleshooting this antibody. Please call us toll-free at (800) 457-3801 option 2, or e-mail <[email protected]>. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-05-18

What is the recommended secondary antibody for Western blot when working with human samples?

Asked by: TinTin
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 14
Date published: 2017-02-04
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Rated 5 out of 5 by from Great product we use this antibody for western blot. it works for us.
Date published: 2017-09-27
Rated 5 out of 5 by from The PI3K antibody is good for NCI-H1975 The PI3K antibody is good for NCI-H1975 NSCLC cell line. The antibody is cost -efficient. I found it from many papers.
Date published: 2017-09-01
Rated 4 out of 5 by from good feature I used this antibody for WB, and it worek very well.
Date published: 2017-07-18
Rated 5 out of 5 by from great I received this antibody a month ago.I used it for western blot.The results were great.
Date published: 2017-07-18
Rated 5 out of 5 by from Popular choice! This is a popular choice for detection of PI 3-kinase p110 in Western blotting and immunoprecipitation applications as indicated by the over 15 product citations.
Date published: 2017-01-18
Rated 5 out of 5 by from great antibody This antibody has great specificity .Clean band can be observed in Western Blot analysis of PI 3-kinase expression in HeLa cell protein.
Date published: 2017-01-18
Rated 5 out of 5 by from Correct MW band in human MG Correct MW band in human MG-63 cells. -SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Worked well in IP and WB in human PC Worked well in IP and WB in human PC-3, LNCaP and DU145 cell lines. -SCBT Publication Review
Date published: 2015-03-18
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