Phosphatase Inhibitor Cocktail BAn inhibitor of PTP-ases as well as acid and alkaline phosphatases

Phosphatase Inhibitor Cocktail B

Phosphatase Inhibitor Cocktail B is rated 4.5 out of 5 by 4.
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Synonym: Phosphatase Inhibitor Cocktail II
Application: An inhibitor of PTP-ases as well as acid and alkaline phosphatases
* Refer to Certificate of Analysis for lot specific data (including water content).
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Phosphatase Inhibitor Cocktail B is a cocktail of five phosphatase inhibitors which has been optimized for the inhibition of protein tyrosine phosphatases, as well as acid and alkaline phosphatases. It can be used on animal tissues, A431, or Jurkat cell extracts. Suitable for cell extracts containing detergents.
Each vial (reconstituted in 1ml) contains 200 mM Imidazole, 100 mM Sodium Fluoride, 115 mM Sodium Molybdate, 100 mM Sodium Orthovanadate and 400 mM Sodium Tartrate Dihydrate. Because this product is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.


References

1. Beynon, R. J., et al. (1989). Proteolytic Enzymes A Practical Approach. p.207. 2. Jain, M. K., et al. (1982). Handbook of Enzyme Inhibitors. John Wiley and Sons, New York, NY. pp. 189-90.

Usage :
Reconstitute each vial with 1 ml H2O to obtain a 1 ml stock solution. Recommended usage is a 100-fold dilution.
Physical State :
Solid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Phosphatase Inhibitor Cocktail B  Product Citations

See how others have used Phosphatase Inhibitor Cocktail B. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 37 total

PMID: # 29725905  Ramírez-Sánchez, J.|Pires, ENS.|Meneghetti, A.|Hansel, G.|Nuñez-Figueredo, Y.|Pardo-Andreu, GL.|Ochoa-Rodríguez, E.|Verdecia-Reyes, Y.|Delgado-Hernández, R.|Salbego, C.|Souza, DO.| et al. 2019. Mol. Neurobiol. 56: 502-512.

PMID: # 31054079  Felices, MJ.|Escusol, S.|Martinez-Beamonte, R.|Gascón, S.|Barranquero, C.|Sanchez-de-Diego, C.|Osada, J.|Rodríguez-Yoldi, MJ.| et al. 2019. J. Physiol. Biochem.

PMID: # 31099942  Xu, C.|Kasimumali, A.|Guo, X.|Lu, R.|Xie, K.|Zhu, M.|Qian, Y.|Chen, X.|Pang, H.|Wang, Q.|Fan, Z.|Dai, H.|Mou, S.|Ni, Z.|Gu, L.| et al. 2019. Nephrology (Carlton).

PMID: # 31046517  Sharlo, K.|Paramonova, I.|Turtikova, O.|Tyganov, S.|Shenkman, B.| et al. 2019. J. Appl. Physiol. 126: 1769-1781.

PMID: # 30866432  Tyganov, S.|Mirzoev, T.|Shenkman, B.| et al. 2019. Int J Mol Sci. 20:

PMID: # 31167138  Gil-Ranedo, J.|Gonzaga, E.|Jaworek, KJ.|Berger, C.|Bossing, T.|Barros, CS.| et al. 2019. Cell Rep. 27: 2921-2933.e5.

PMID: # 30007036  Aranda, CJ.|Ocón, B.|Arredondo-Amador, M.|Suárez, MD.|Zarzuelo, A.|Chazin, WJ.|Martínez-Augustin, O.|Sánchez de Medina, F.| et al. 2018. Br. J. Pharmacol. 175: 3797-3812.

PMID: # 30135215  Takada, M.|Hix, JML.|Corner, S.|Schall, PZ.|Kiupel, M.|Yuzbasiyan-Gurkan, V.| et al. 2018. Mol. Cancer Ther. 17: 2439-2450.

PMID: # 29409958  Agasid, MT.|Wang, X.|Huang, Y.|Janczak, CM.|Bränström, R.|Saavedra, SS.|Aspinwall, CA.| et al. 2018. Protein Expr. Purif. 146: 61-68.

PMID: # 28839114  Belova, SP. et al. 2017. Physiol Rep. 5:

Citations 1 to 10 of 37 total
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Rated 3 out of 5 by from Works well for WB Very economical to use with clean WB results. Lasts for ever as very minimal quantity is needed in the lysis buffer
Date published: 2019-03-14
Rated 5 out of 5 by from Great product I bought that items and so happy with the performance and results
Date published: 2017-03-07
Rated 5 out of 5 by from I use the Phosphatase Inhibitor Cocktail I use the Phosphatase Inhibitor Cocktail B together with Phosphatase Inhibitor Cocktail A (45044) on my cell lysates. The inhibition works very well! I can clearly detect phosphorylation on my Western Blots
Date published: 2015-10-13
Rated 5 out of 5 by from By having this Phosphatase Inhibitor Cocktail By having this Phosphatase Inhibitor Cocktail B present in the cell lysate, de-phosphorylation is prevented by any phosphatase enzymes that may be present in the sample. This action is demonstrated by treating K-562 cell lysate (containing this inhibitor as part of the lysis buffer) with lambda protein phosphatase to see if the phosphorylation of the Cofilin 1 protein can be altered. By WB, we can see that this inhibitor cocktail does indeed prevent lambda protein phosphatase from de-phosphorylating p-Cofilin 1 protein. -SCBT QC
Date published: 2015-01-27
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