PHC2 Double Nickase Plasmid (h): sc-407705-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PHC2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PHC2 Double Nickase Plasmid (h) and PHC2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PHC2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHC2 Double Nickase Plasmid (h) | sc-407705-NIC | 20 µg | $410.00 | |||
PHC2 Double Nickase Plasmid (h2) | sc-407705-NIC-2 | 20 µg | $410.00 |
PHC2 encodes Polyhomeotic homolog 2, a core subunit of Polycomb repressive complex 1 (PRC1) that helps maintain stable transcriptional silencing through chromatin compaction and H2A lysine 119 ubiquitination. By contributing to epigenetic memory, PHC2 supports control of developmental gene expression programs, lineage specification, and long-term regulation of cell identity. Dysregulation of Polycomb group proteins, including PHC2, is linked to altered differentiation states and aberrant proliferation, making PHC2 a relevant target for studies of chromatin-based gene regulation. PHC2 is therefore commonly investigated in the context of transcriptional repression networks and PRC1-dependent pathways that modulate genome organization.
PHC2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PHC2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PHC2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PHC2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PHC2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.