Phalloidin-tetramethylrhodamine conjugate

Phalloidin-tetramethylrhodamine conjugate

Phalloidin-tetramethylrhodamine conjugate is rated 5.0 out of 5 by 1.
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Application: A fluorescent phallotoxin that can be used to identify filamentous actin
Molecular Weight: ~1300
Molecular Formula: C60H70O14N11S
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Phalloidin-Tetramethylrhodamine Conjugate is a fluorescent phalloidin (sc-202763) derivative that can be used to identify filamentous actin. It is a superior replacement to Phalloidin-Tetramethylrhodamine B isothiocyanate (sc-301530) because of the amide conjugation, which results in a more stable conjugate. It binds polymeric F actin, stabilizing it and interfering with the function of actin-rich structures. The rhodamine moiety then allows for visualization of F-actin through fluorescence. The dye excites at a wavelength of 546 nm and emits a wavelength of 575 nm.


References

1. Faulstich, H. et al. 1983. Exp. Cell Res. 144(1): 73-82. PMID: 6840214
2. Waggoner, A. et al. 1989. Methods Cell Biol. 30 : 449-478. PMID: 2648118

Usage :
Sample Prepare 1X CruzFluor Phalloidin conjugate working solution: by adding 1 uL of 1000X Phalloidin CruzFluor stock conjugate DMSO solution to 1 mL of PBS with 1% BSA.



Note 2: Different cell types might be stained differently. The concentration of phalloidin conjugate working
solution should be prepared accordingly.
Formulation :
1000X stock solution in DMSO; 30 µl total
Physical State :
Liquid
Solubility :
Soluble in DMSO, and water (low).
Storage :
Desiccate at -20° C
Fluoresence :
λex 546 nm, λem 575 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Transport :
UN 2811, Class 6.1, Packing group II

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Certificate of Analysis

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Phalloidin-tetramethylrhodamine conjugate  Product Citations

See how others have used Phalloidin-tetramethylrhodamine conjugate. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 27382603  Liskovykh, M. et al. 2016. Molecular therapy. Methods & clinical development. 3: 16043.

PMID: # 25517094  Boucrot, E. et al. 2015. Nature. 517: 460-5.

PMID: # 25795513  Li, H. et al. 2015. Free radical biology & medicine. 84: 311-21.

PMID: # 24810525  Xu, J. et al. 2014. Critical care medicine. 42: e583-94.

Citations 1 to 4 of 4 total
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Rated 5 out of 5 by from Various publications cite the use of Phalloidin Various publications cite the use of Phalloidin-Tetramethylrhodamine Conjugate, a fluorescent phalloidin (sc-202763) derivative, to identify filamentous actin. The dye excites at a wavelength of 546 nm and emits a wavelength of 575 nm. -SCBT Publication Review
Date published: 2015-06-16
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