Phalloidin CruzFluor™ 647 Conjugate is a deep red fluorescent conjugate that is an excellent substitute for AlexFluor® 647. Phalloidin is a heptapeptide phallotoxin produced by Amanita phalloides, the death cap mushroom. Phalloidin binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.
These properties of Phalloidin make Phalloidin CruzFluor™ Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors the series is excellent for multicolor imaging applications.
See how others have used Phalloidin CruzFluor™ 647 Conjugate. Click on the entry to view the PubMed entry .
PMID: # 33824207 Diggins, NL.|Crawford, LB.|Hancock, MH.|Mitchell, J.|Nelson, JA.| et al. 2021. mBio. 12:
PMID: # 33571402 Zeigler, AC.|Chandrabhatla, AS.|Christiansen, SL.|Nelson, AR.|Holmes, JW.|Saucerman, JJ.| et al. 2021. CPT Pharmacometrics Syst Pharmacol.
PMID: # 28528863 Inostroza-Brito, KE.|Collin, EC.|Majkowska, A.|Elsharkawy, S.|Rice, A.|Del Río Hernández, AE.|Xiao, X.|Rodríguez-Cabello, J.|Mata, A.| et al. 2017. Acta Biomater. 58: 80-89.
PMID: # 28065854 Sušjan, P.|Roškar, S.|Hafner-Bratkovič, I.| et al. 2017. Biochem. Biophys. Res. Commun. 483: 823-828.
PMID: # 26438793 Carrasco, SE. et al. 2015. Infect. Immun. 83: 4848-60.
PMID: # 24623484 Marques, JG. et al. 2014. Pharm. Res. -.
PMID: # 24434137 Dev Cell. 70-80.
PMID: # 31920691 Front Physiol. 1481.
PMID: # 28767724 PLoS One. e0181579.