Phalloidin CruzFluor™ 633 Conjugate MW: ~2100

Phalloidin CruzFluor™ 633 Conjugate

Phalloidin CruzFluor 633 Conjugate is rated 4.0 out of 5 by 2.
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Alternate Names: Phalloidin CruzFluor™ 633 Conjugate is known as a conjugated, high-affinity F-actin probe.
Application: Phalloidin CruzFluor™ 633 Conjugate is a fluorescent phalloidin conjugate that selectively binds to F-actins.
Molecular Weight: ~2100
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Phalloidin CruzFluor™ 633 Conjugate is a deep red fluorescent conjugate that is an excellent substitute for AlexaFluor 633. Phalloidin is a heptapeptide phallotoxin produced by Amanita phalloides, the death cap mushroom. Phalloidin binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.
These properties of Phalloidin make Phalloidin CruzFluor™ 633 Conjugate Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ 633 Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors, the series is excellent for multicolor imaging applications.


References

1. Niedergang F, Colucci-Guyon E, Dubois T, Raposo G, Chavrier P
J Cell Biol (2003) 161:1143-1143
2. Arakawa Y, Bito H, Furuyashiki T, Tsuji T, Takemoto-Kimura S, Kimura K, Nozaki K, Hashimoto N, Narumiya S
J Cell Biol (2003) 161:381-391
3. Rosado M, Barber CF, Berciu C, Feldman S, Birren SJ, Nicastro D, Goode BL,
Mol Biol Cell (2014) 25:811-827

Usage :
Phalloidin CruzFluor™ conjugates can be used alongside antibodies in IF staining of formalin-fixed cells and formalin-fixed, frozen tissue sections. The Phalloidin will stain F-actin.

1. Prepare 1000 X Phalloidin DMSO stock solution: by adding 30 uL of DMSO into the powder form vials.

2. Prepare 1X Phalloidin conjugate working solution: by adding 1 uL of 1000X Phalloidin conjugate DMSO solution to 1 mL of PBS with 1% BSA

Note 1: The unused 1000X DMSO stock solution of Phalloidin conjugate should be aliquoted and stored at -20° C. protected from light.

Note 2: Different cell types might be stained differently. The concentration of Phalloidin conjugate working solution should be prepared accordingly.


3. Stain the cells or tissue:

3.1 Perform formaldehyde fixation. Incubate cells with 3.0-4.0% formaldehyde in PBS at room temperature for 10-30 minutes.

Note 3: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.

3.2 Rinse the fixed cells 2-3 times in PBS.

3.3 Optional: Add 0.1% Triton X-100 in PBS into fixed cells (from Step 3.2) for 3 to 5 minutes to increase permeability. Rinse the cells 2-3 times in PBS.

3.4 Add Phalloidin conjugate working solution (from Step 2) into the fixed cells (from Step 3.2 or 3.3), and stain the cells at room temperature for 20 to 90 minutes.

3.5 Rinse cells gently with PBS 2-3 times to remove excess Phalloidin conjugate before mounting, sealing and imaging under microscope.

Note 4: If antibody staining is desired, perform blocking, primary antibody and secondary antibody incubations separately between Steps 3.3 and 3.4 according to manufacturer′s protocol. Labeling with Phalloidins can be combined with secondary antibody staining, as long as the correct dilutions of each reagent are used in the staining solution.

Note 5: Paraffin-embedded tissues are not recommended for Phalloidin staining, because the use of solvents during the deparaffinization process will disrupt actin.
Formulation :
≤ 100% phalloidin
Physical State :
Solid
Solubility :
Soluble in DMSO.
Storage :
Store at -20° C
Fluoresence :
λex 634 nm, λem 649 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Phalloidin CruzFluor™ 633 Conjugate  Product Citations

See how others have used Phalloidin CruzFluor™ 633 Conjugate. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 11 total

PMID: # 33393462  Mykytyn, AZ.|Breugem, TI.|Riesebosch, S.|Schipper, D.|van den Doel, PB.|Rottier, RJ.|Lamers, MM.|Haagmans, BL.| et al. 2021. Elife. 10:

PMID: # 33283287  Lamers, MM.|van der Vaart, J.|Knoops, K.|Riesebosch, S.|Breugem, TI.|Mykytyn, AZ.|Beumer, J.|Schipper, D.|Bezstarosti, K.|Koopman, CD.|Groen, N.|Ravelli, RBG.|Duimel, HQ.|Demmers, JAA.|Verjans, GMGM.|Koopmans, MPG.|Muraro, MJ.|Peters, PJ.|Clevers, H.|Haagmans, BL.| et al. 2020. EMBO J. e105912.

PMID: # 31461652  2019. Cell Rep. 28: 2358-2372.e6.

PMID: # 30537732  Lambertini, E.|Penolazzi, L.|Angelozzi, M.|Bergamin, LS.|Manferdini, C.|Vieceli Dalla Sega, F.|Paolella, F.|Lisignoli, G.|Piva, R.| et al. 2018. Cell. Physiol. Biochem. 51: 2237-2249.

PMID: # 26130066  Carlson, CG. et al. 2016. J. Cell. Physiol. 231: 788-97.

PMID: # 26377855  Chen, Q. et al. 2015. Adv Healthc Mater. 4: 2291-6.

PMID: # 23559100  Yamazaki, O. et al. 2013. Pflugers Arch. 465: 1281-1291.

PMID: # 26548584  Analyst. 661-8.

PMID: # 30962439  Nat Commun. 1608.

PMID: # 32585813  Int J Mol Sci.

Citations 1 to 10 of 11 total

Does anybody knows if this product works on whole mounts of drosophila larval brain?

Asked by: Maribel
Thank you for your message. Please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27
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Rated 4 out of 5 by from Good product I received this a month ago and the product is really really good for imaging.
Date published: 2016-12-19
Rated 4 out of 5 by from Strong red fluorescence staining was observed Strong red fluorescence staining was observed by IF following incubation of formalin fixed HeLa cells with a F-actin antibody conjugated to Phalloidin CruzFluor 633 Conjugate. Crisp red fluorescence observed. -SCBT QC
Date published: 2015-02-07
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