Phalloidin CruzFluor™ 555 Conjugate is an orange fluorescent conjugate that is an excellent substitute for AlexFluor® 555. Phalloidin CruzFluor™ 555 Conjugate is a heptapeptide phallotoxin produced by Amanita phalloides, the death cap mushroom. Phalloidin CruzFluor™ 555 Conjugate binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.
These properties of Phalloidin CruzFluor™ 555 Conjugate make Phalloidin CruzFluor™ Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors the series is excellent for multicolor imaging applications. Phalloidin CruzFluor™ 555 Conjugate is also known as Phalloidin-iFluor™ 555 Conjugate.
See how others have used Phalloidin CruzFluor™ 555 Conjugate. Click on the entry to view the PubMed entry .
PMID: # 30746686 2019. J. Cell. Physiol.
PMID: # 29470914 ACS Appl Mater Interfaces. 8507-8518.
PMID: # 32924006 Mater Today Bio. 100071.
PMID: # 33001475 EMBO J. e103530.
PMID: # 32990682 J Clin Invest.
PMID: # 30156819 ACS Appl Mater Interfaces. 31168-31177.