Phalloidin CruzFluor 350 Conjugate MW: ~1000

Phalloidin CruzFluor™ 350 Conjugate

Phalloidin CruzFluor 350 Conjugate is rated 5.0 out of 5 by 1.
  • y_2021, m_9, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_363789, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 128ms
  • REVIEWS, PRODUCT
loading
5
1
4
0
3
0
2
0
1
0
Application: Phalloidin CruzFluor™ 350 Conjugate is a fluorescent phalloidin conjugate that selectively binds to F-actins
Molecular Weight: ~1000
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
loading

Phalloidin CruzFluor™ 350 Conjugate is a blue fluorescent dye. Phalloidin is a heptapeptide phallotoxin produced by Amanita phalloides, the death cap mushroom. Phalloidin binds and stabilizes filamentous F-actin, preventing the dissociation of paired filaments and disturbing the natural equilibrium between filamentous (polymeric) and globular (monomeric) actin in the cell, as well as inhibit the ATP hydrolysis activity of F-actin. Actin is used by the cell for mechanical processes such as growth, scaffolding of the cytoskeleton, and locomotion, and disruption of this actin equilibrium destroys cellular functioning. The magnitude by which the actin protein differs from species to species is no more than 20%.
These properties of Phalloidin make Phalloidin CruzFluor™ Conjugates a fantastic tool for labeling, identifying and quantitating F-actins. The Phalloidin CruzFluor™ Conjugates are used at nanomolar concentrations with cell-free experiments, cell culture experiments, formaldehyde-fixed, permeabilized tissue sections and the study of actin networks. Because Phalloidin CruzFluor™ Conjugates are offered in different colors the series is excellent for multicolor imaging applications.

Usage :
Phalloidin CruzFluor™ conjugates can be used alongside antibodies in IF staining of formalin-fixed cells and formalin-fixed, frozen tissue sections. The Phalloidin will stain F-actin.

1. Prepare 1000 X Phalloidin DMSO stock solution: by adding 30 uL of DMSO into the powder form vials.

2. Prepare 1X Phalloidin conjugate working solution: by adding 1 uL of 1000X Phalloidin conjugate DMSO solution to 1 mL of PBS with 1% BSA

Note 1: The unused 1000X DMSO stock solution of Phalloidin conjugate should be aliquoted and stored at -20° C. protected from light.

Note 2: Different cell types might be stained differently. The concentration of Phalloidin conjugate working solution should be prepared accordingly.


3. Stain the cells or tissue:

3.1 Perform formaldehyde fixation. Incubate cells with 3.0-4.0% formaldehyde in PBS at room temperature for 10-30 minutes.

Note 3: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.

3.2 Rinse the fixed cells 2-3 times in PBS.

3.3 Optional: Add 0.1% Triton X-100 in PBS into fixed cells (from Step 3.2) for 3 to 5 minutes to increase permeability. Rinse the cells 2-3 times in PBS.

3.4 Add Phalloidin conjugate working solution (from Step 2) into the fixed cells (from Step 3.2 or 3.3), and stain the cells at room temperature for 20 to 90 minutes.

3.5 Rinse cells gently with PBS 2-3 times to remove excess Phalloidin conjugate before mounting, sealing and imaging under microscope.

Note 4: If antibody staining is desired, perform blocking, primary antibody and secondary antibody incubations separately between Steps 3.3 and 3.4 according to manufacturer′s protocol. Labeling with Phalloidins can be combined with secondary antibody staining, as long as the correct dilutions of each reagent are used in the staining solution.

Note 5: Paraffin-embedded tissues are not recommended for Phalloidin staining, because the use of solvents during the deparaffinization process will disrupt actin.
Formulation :
1000X stock solution in DMSO; 30 µl total
Physical State :
Liquid
Solubility :
Soluble in DMSO, and water (low).
Storage :
Store at -20° C
Fluoresence :
λex 353 nm, λem 442 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Phalloidin CruzFluor 350 Conjugate  Product Citations

See how others have used Phalloidin CruzFluor 350 Conjugate. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 33388318  Zhao, Y.|Schoeps, B.|Yao, D.|Zhang, Z.|Schuck, K.|Tissen, V.|Jäger, C.|Schlitter, AM.|van der Kammen, R.|Ludwig, C.|D'Haese, JG.|Raulefs, S.|Maeritz, N.|Shen, S.|Zou, X.|Krüger, A.|Kleeff, J.|Michalski, CW.|Friess, H.|Innocenti, M.|Kong, B.| et al. 2021. Gastroenterology. 160: 1755-1770.e17.

Citations 1 to 1 of 1 total
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 97ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 5 out of 5 by from Bright blue staining observed by IF when Bright blue staining observed by IF when conjugating this Phalloidin CruzFluor 350 Conjugate to an antibody targeting F-actin (cells used - formalin fixed HepG2). - SCBT QC
Date published: 2015-01-09
  • y_2021, m_9, d_19, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_363789, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT