Perlecan Antibody (E-6)recommended for detection of Perlecan of human origin by WB, IP, IF, IHC(P) and ELISA

Perlecan Antibody (E-6): sc-377219

Perlecan Antibody (E-6) is rated 4.5 out of 5 by 4.
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Datasheets

    • Perlecan Antibody (E-6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 4092-4391 mapping near the C-terminus of Perlecan of human origin
    • Perlecan Antibody (E-6) is recommended for detection of Perlecan of human origin by WB, IP, IF, IHC(P) and ELISA
    • Perlecan Antibody (E-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Perlecan (E-6): sc-377219.
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Can Perlecan (E-6): sc-377219 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Perlecan (E-6): sc-377219 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good antibody antibody clean and specific - tested on human primary muscle cells - IF
Date published: 2018-04-04
Rated 5 out of 5 by from Works perfectly for IHC-P Stained at 1:50 (IHC-P) for cells embedded in a gel and it worked perfectly fine.
Date published: 2018-02-21
Rated 4 out of 5 by from works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 5 out of 5 by from Nice Western blot data of Perlecan expression Nice Western blot data of Perlecan expression in HeLa nuclear extract. -SCBT QC
Date published: 2014-02-13
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