Anti-CD31/PECAM-1 Antibody (JC70) recommended for detection of PECAM-1 of human origin by WB, IP, IF, IHC(P) and FCM

Anti-CD31/PECAM-1 Antibody (JC70): sc-53411

Anti-CD31/PECAM-1 Antibody (JC70) is rated 4.9 out of 5 by 9.
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Datasheets

    • CD31/PECAM-1 Antibody (JC70) is a mouse monoclonal IgG1 κ, cited in 29 publications, provided at 200 µg/ml
    • raised against spleen of human origin
    • CD31/PECAM-1 Antibody (JC70) is recommended for detection of PECAM-1 of human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-CD31/PECAM-1 Antibody (JC70) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD31/PECAM-1 (JC70): sc-53411.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD31/PECAM-1 Antibody (JC70) for WB and IHC(P) applications. These reagents are now offered in bundles with CD31/PECAM-1 Antibody (JC70) (see ordering information below).
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Is the epitope of this antibody extracellular?

Asked by: Luong
Thank you for your question. PECAM-1 Antibody (JC70): sc-53411 is raised against spleen of human origin. The exact epitope recognized by this mouse monoclonal antibody has not been determined, thus we do not know if it is extracellular or intracellular.
Answered by: Technical Support
Date published: 2017-04-13

Can PECAM-1 (JC70): sc-53411 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, PECAM-1 (JC70): sc-53411 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent antibody This antibody works perfectly on human aortic endothelial cells. Our lab use this antibody to verify the phenotype of endothelial cells that we isolate ourselves from human aortic specimens. We incubate PFA-fixed cells with the primary antibody overnight at 4C (dilution 1/100) and obtain the expected staining pattern with fluorescence at the plasma membrane.
Date published: 2018-03-01
Rated 5 out of 5 by from Good I was gifted friendly by other lab. and proceeded IF with a dilution factor of 1:50 in HUVEC as a positive control. It was good. The higher dilution factor also will be good I think.
Date published: 2017-02-12
Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of PECAM-1 expression in Jurkat, CCRF-CEM and K-562 whole cell lysates.
Date published: 2017-01-26
Rated 5 out of 5 by from It has been reported that this antibody was It has been reported that this antibody was used successfully in immunocytochemistry with human ubendocardial MSCs. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Worked well in immunohistochemistry using Worked well in immunohistochemistry using formalin fixed human Jelly Mesenchymal Stem Cells. -SCBT Publication Review
Date published: 2015-04-12
Rated 5 out of 5 by from Publishable data obtained by flow cytometry Publishable data obtained by flow cytometry using human mononuclear cells. -SCBT Publication Review
Date published: 2015-01-13
Rated 5 out of 5 by from Produced good quality flow cytometry data Produced good quality flow cytometry data using human stem cells. -SCBT Publication Review
Date published: 2015-01-05
Rated 4 out of 5 by from Difference states of post Difference states of post-translational modification observed by Western Blot using this PECAM-1 antibody with Jurkat, CCRF-CEM and K-562 whole cell lysates. -SCBT QC
Date published: 2015-01-05
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