Anti-CD31/PECAM-1 Antibody (ER-MP12) recommended for detection of PECAM-1 of mouse origin by IF, IHC(P) and FCM

Anti-CD31/PECAM-1 Antibody (ER-MP12): sc-52713

Anti-CD31/PECAM-1 Antibody (ER-MP12) is rated 4.0 out of 5 by 1.
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Datasheets

    • CD31/PECAM-1 Antibody (ER-MP12) is a rat monoclonal IgG2a, cited in 5 publications, provided at 200 µg/ml
    • raised against macrophage precursor cells of mouse origin
    • recommended for detection of PECAM-1 of mouse origin by IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD31/PECAM-1 (H-3): sc-376764 for CD31/PECAM-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD31/PECAM-1 Antibody (ER-MP12). This work is in progress.
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How can PECAM-1 (ER-MP12): sc-52713 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Germaine
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-52713 PE) or fluoroscein (sc-52713 FITC).
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Publishable data obtained by immunocytochemistry Publishable data obtained by immunocytochemistry using mouse glomeruli samples. -SCBT Publication Review
Date published: 2013-09-06
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