Anti-CD31/PECAM-1 Antibody (E-8) recommended for detection of PECAM-1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CD31/PECAM-1 Antibody (E-8): sc-133091

Anti-CD31/PECAM-1 Antibody (E-8) is rated 5.0 out of 5 by 4.
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Datasheets

    • CD31/PECAM-1 Antibody (E-8) is a mouse monoclonal IgG1 κ, cited in 5 publications, provided at 200 µg/ml
    • raised against amino acids 28-327 mapping at the N-terminus of PECAM-1 of human origin
    • recommended for detection of PECAM-1 of human origin by WB, IP, IF, IHC(P) and ELISA
    • See CD31/PECAM-1 (H-3): sc-376764 for CD31/PECAM-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD31/PECAM-1 Antibody (E-8) for WB and IHC(P) applications. These reagents are now offered in bundles with CD31/PECAM-1 Antibody (E-8) (see ordering information below).
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I am using PECAM-1 (E-8): sc-133091 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Publishable IHC data in human kidney cancer Publishable IHC data in human kidney cancer cell line 786-O. -SCBT Publication Review
Date published: 2015-03-28
Rated 5 out of 5 by from IF publication with human HUVECs IF publication with human HUVECs. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Good specificity and signal strength in Jurkat Good specificity and signal strength in Jurkat, human PBL and THP-1 whole cell lysates. -SCBT QC
Date published: 2014-07-14
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