Anti-CD31/PECAM-1 Antibody (3H1217) recommended for detection of PECAM-1 of mouse and rat origin by IF, IHC(P) and FCM

Anti-CD31/PECAM-1 Antibody (3H1217): sc-71873

Anti-CD31/PECAM-1 Antibody (3H1217) is rated 4.0 out of 5 by 1.
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Datasheets

    • CD31/PECAM-1 Antibody (3H1217) is a mouse monoclonal IgG1, cited in 11 publications, provided at 200 µg/ml
    • raised against activated microglial cells of Lewis rat origin
    • recommended for detection of PECAM-1 of mouse and rat origin by IF, IHC(P) and FCM
    • available conjugated to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • See CD31/PECAM-1 (H-3): sc-376764 for CD31/PECAM-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD31/PECAM-1 Antibody (3H1217). This work is in progress.
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Hey I am looking for some free samples of antibodies and I would like to know how to go about ordering for the same.

Asked by: Shikha
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

I am using PECAM-1 (3H1217): sc-71873 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Publishable data by immunohistochemistry Publishable data by immunohistochemistry using formalin fixed, paraffin embedded rat heart tissue. -SCBT Publication Review
Date published: 2014-05-19
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