Anti-PDGF-D Antibody (E-6) recommended for detection of PDGF-D of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-PDGF-D Antibody (E-6): sc-137031

Anti-PDGF-D Antibody (E-6) is rated 5.0 out of 5 by 1.
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Datasheets

    • PDGF-D Antibody (E-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 131-270 mapping within an internal region of PDGF-D of human origin
    • recommended for detection of PDGF-D of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PDGF-D Antibody (E-6) for WB applications. These reagents are now offered in bundles with PDGF-D Antibody (E-6) (see ordering information below).
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How much sample do you recommend loading on the gel for Western blot detection using PDGF-D (E-6): sc-137031?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Satisfactory Western blot data of PDGF Satisfactory Western blot data of PDGF-D expression in non-transfected and truncated mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-03-22
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