Anti-PD-ECGF Antibody (PGF-44C) recommended for detection of PD-ECGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-PD-ECGF Antibody (PGF-44C): sc-47702

Anti-PD-ECGF Antibody (PGF-44C) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-PD-ECGF Antibody (PGF-44C) is a mouse monoclonal IgG1 κ PD-ECGF antibody, cited in 16 publications, provided at 200 µg/ml
    • raised against full length recombinant PD-ECGF of human origin
    • Anti-PD-ECGF Antibody (PGF-44C) is recommended for detection of PD-ECGF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PD-ECGF Antibody (PGF-44C) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PD-ECGF (PGF-44C): sc-47702.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PD-ECGF Antibody (PGF-44C) for WB and IHC(P) applications. These reagents are now offered in bundles with PD-ECGF Antibody (PGF-44C) (see ordering information below).
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What blocking solution should I use with PD-ECGF (PGF-44C): sc-47702 monoclonal antibody for Western blot?

Asked by: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of PD-ECGF expression in MDA-MB-231, MDA-MB-468, MCF7 and SK-BR-3 whole cell lysates.
Date published: 2017-02-03
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