PCAF Antibody (E-8)recommended for detection of PCAF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PCAF Antibody (E-8): sc-13124

PCAF Antibody (E-8) is rated 4.9 out of 5 by 12.
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Datasheets

    • PCAF Antibody (E-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 464-832 of PCAF of human origin
    • PCAF Antibody (E-8) is recommended for detection of PCAF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with GCN5
    • PCAF Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCAF (E-8): sc-13124.
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Can PCAF Antibody (E-8): sc-13124 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Yes, PCAF Antibody (E-8): sc-13124 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from Recommended for western blotting! It detected endogenous PCAF in lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween-20, overnight at 4C).
Date published: 2018-06-18
Rated 5 out of 5 by from Great specificity! The antibody to PCAF (E-8) is great to do Western blot, and the results show a very clear background.
Date published: 2018-02-25
Rated 5 out of 5 by from excellent tested on rat tissue samples in western blot and worked perfectly.
Date published: 2017-12-13
Rated 4 out of 5 by from good antibody I received it as a sample product and it worked well.
Date published: 2017-07-25
Rated 5 out of 5 by from Overall great antibody Excellent, well-published antibody for PCAF in mouse, rat, and human samples. Strong, specific signal observed in multiple applications.
Date published: 2017-01-16
Rated 5 out of 5 by from Great features I bought this antibody two months age, and I am very glad that this antibody is very efficient. I uesd PCAF of 1:500 dilution in Wb, second antibody is 1:4000. loaded ~50 ug. The band is clearly.
Date published: 2016-11-23
Rated 5 out of 5 by from Good specificity in WB in 293 cells Good specificity in WB in 293 cells. -SCBT QC
Date published: 2015-05-31
Rated 5 out of 5 by from Strong pulldown in IP in human T3 Strong pulldown in IP in human T3-1 cells. -SCBT Publication Review
Date published: 2015-05-19
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