Anti-Pbx 1b Antibody (41.1) recommended for detection of Pbx 1b of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Pbx 1b Antibody (41.1): sc-101852

Anti-Pbx 1b Antibody (41.1) is rated 3.5 out of 5 by 4.
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Datasheets

    • Anti-Pbx 1b Antibody (41.1) is a mouse monoclonal IgG1 κ Pbx 1b antibody, cited in 5 publications, provided at 200 µg/ml
    • raised against a recombinant protein corresponding to amino acids 319-347 of Pbx 1b of human origin
    • Anti-Pbx 1b Antibody (41.1) is recommended for detection of Pbx 1b of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Pbx 1b Antibody (41.1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pbx 1b (41.1): sc-101852.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Pbx 1b Antibody (41.1) for WB and IHC(P) applications. These reagents are now offered in bundles with Pbx 1b Antibody (41.1) (see ordering information below).
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I am using Pbx 1b (41.1): sc-101852 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from no signal in IF in brain I didn't detect staining signal in 4% PFA fixed embryonic mouse brain sections.
Date published: 2018-01-18
Rated 5 out of 5 by from High Quality Antibody The Pbx 1b Antibody (41.1): sc-101852 provides excellent data for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-02-03
Rated 4 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-04-12
Rated 4 out of 5 by from IF publishable data with human MCF7 cell line IF publishable data with human MCF7 cell line - SCBT Publication Review
Date published: 2014-03-30
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