Anti-Pbx 1/2/3/4 Antibody (F-3) recommended for detection of Pbx 1, Pbx 2, Pbx 3 and Pbx 4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-Pbx 1/2/3/4 Antibody (F-3): sc-28313

Anti-Pbx 1/2/3/4 Antibody (F-3) is rated 4.3 out of 5 by 3.
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Datasheets

    • Anti-Pbx 1/2/3/4 Antibody (F-3) is a mouse monoclonal IgG1 κ Pbx 1/2/3/4 antibody, cited in 6 publications, provided at 200 µg/ml
    • raised against amino acids 1-260 of Pbx 1 of human origin
    • Anti-Pbx 1/2/3/4 Antibody (F-3) is recommended for detection of Pbx 1, Pbx 2, Pbx 3 and Pbx 4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Pbx 1/2/3/4 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-28313 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pbx 1/2/3/4 (F-3): sc-28313.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for Pbx 1/2/3/4 Antibody (F-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Pbx 1/2/3/4 Antibody (F-3) (see ordering information below).
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I am using Pbx 1/2/3/4 (F-3): sc-28313 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB -SCBT QC
Date published: 2015-07-15
Rated 4 out of 5 by from Nice bands seen in WB using Jurkat nuclear Nice bands seen in WB using Jurkat nuclear extract and BJAB whole cell lysate. -SCBT QC
Date published: 2015-06-08
Rated 5 out of 5 by from Produced superb immunofluorescence nuclear Produced superb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-13
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