PABPC4 Double Nickase Plasmid (h): sc-411206-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PABPC4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PABPC4 Double Nickase Plasmid (h) and PABPC4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PABPC4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PABPC4 Double Nickase Plasmid (h) | sc-411206-NIC | 20 µg | $410.00 | |||
PABPC4 Double Nickase Plasmid (h2) | sc-411206-NIC-2 | 20 µg | $410.00 |
PABPC4 (poly(A)-binding protein cytoplasmic 4) is an RNA-binding protein that associates with the poly(A) tails of mature mRNAs to regulate transcript stability, translation initiation, and mRNA turnover in the cytoplasm. By engaging core messenger ribonucleoprotein (mRNP) complexes and interacting with translation factors, PABPC4 helps coordinate post-transcriptional gene expression programs that shape cellular activation and stress responses. PABPC4 has been studied in the context of hematopoietic and immune-related biology, where altered mRNA processing and translation control can contribute to dysregulated proliferation and inflammatory signaling. Perturbation of PABPC4-dependent mRNP dynamics is also relevant for investigating disease mechanisms linked to aberrant RNA metabolism.
PABPC4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PABPC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PABPC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PABPC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PABPC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.