PA28 beta Antibody (G-10)recommended for detection of PA28β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PA28β Antibody (G-10): sc-390563

PA28β Antibody (G-10) is rated 4.5 out of 5 by 2.
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Datasheets

    • PA28β Antibody (G-10) is a mouse monoclonal IgG1 (lambda light chain) provided at 200 µg/ml
    • raised against amino acids 176-239 mapping at the C-terminus of PA28β of human origin
    • PA28 beta Antibody (G-10) is recommended for detection of PA28β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
    • PA28 beta Antibody (G-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PA28β (G-10): sc-390563.
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Can PA28β (G-10): sc-390563 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, PA28β (G-10): sc-390563 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Work for WB Use siPA28β-MEF cell samples, Ab is diluted to 1:500, see the clear and strong band.
Date published: 2017-08-17
Rated 5 out of 5 by from Satisfactory Western Blot data of PA28 expression Satisfactory Western Blot data of PA28 expression in HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2014-09-21
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