p54/nrb Antibody (G-1)recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

p54/nrb Antibody (G-1): sc-376865

p54/nrb Antibody (G-1) is rated 4.8 out of 5 by 5.
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Datasheets

    • p54/nrb Antibody (G-1) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 381-465 mapping near the C-terminus of p54/nrb of human origin
    • p54/nrb Antibody (G-1) is recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • p54/nrb Antibody (G-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p54/nrb (G-1): sc-376865.
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Can you recommend a positive control to use with p54/nrb (G-1): sc-376865 monoclonal antibody for Western blot?

Asked by: jenni
We recommend HeLa: sc-2120, MOLT-4: sc-2151, MCF7: sc--2149 or NIH/3T3: sc-2138 nuclear extracts to use as positive controls as we have great Western blot data showing specific detection of p54/nrb in these cell lines.
Answered by: Technical Support 2
Date published: 2017-01-13

Can p54/nrb (G-1): sc-376865 this antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, p54/nrb (G-1): sc-376865 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 2
Date published: 2017-01-12
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Rated 4 out of 5 by from Good antibody for IF, although not so good as A-11 This would be my second choise after A-11 for IF. Staining looks specific.
Date published: 2019-10-18
Rated 5 out of 5 by from Wow !! Superb product Strong western data with little to no background; also great product for IF and IHC staining applications
Date published: 2017-01-30
Rated 5 out of 5 by from Great antibody Strong detection of p54/nrb in WB, IHC, and IF. Great antibody for mouse, rat, and human detection.
Date published: 2017-01-25
Rated 5 out of 5 by from Excellent immunofluorescence nuclear staining Excellent immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-24
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