p54/nrb Antibody (E-3)recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

p54/nrb Antibody (E-3): sc-376806

p54/nrb Antibody (E-3) is rated 5.0 out of 5 by 2.
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Datasheets

    • p54/nrb Antibody (E-3) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 381-465 mapping near the C-terminus of p54/nrb of human origin
    • recommended for detection of p54/nrb of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • TransCruz reagent for ChIP application (sc-376806 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can p54/nrb (E-3): sc-376806 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, p54/nrb (E-3): sc-376806 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Beautiful immunoperoxidase nuclear staining Beautiful immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-01-13
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