Anti-P2Y2 Antibody (E-3) recommended for detection of P2Y2 of and human origin by WB, IP, IF and ELISA

Anti-P2Y2 Antibody (E-3): sc-518019

Anti-P2Y2 Antibody (E-3) is rated 3.0 out of 5 by 1.
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Datasheets

    • Anti-P2Y2 Antibody (E-3) is a mouse monoclonal IgG2a κ P2Y2 antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 308-370 mapping at the C-terminus of P2Y2 of human origin
    • P2Y2 Antibody (E-3) is recommended for detection of P2Y2 of human origin by WB, IP, IF and ELISA
    • Anti-P2Y2 Antibody (E-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of P2Y2 (E-3): sc-518019.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for P2Y2 Antibody (E-3) for WB applications. These reagents are now offered in bundles with P2Y2 Antibody (E-3) (see ordering information below).
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We have noticed some cross -reactivity tissue with mouse inoculated with human-derived tumor cells. We blocked mouse-on-mouse technique but still saw immunopositivity. We only want human reactivity. Has it been proven that this does NOT react with mouse?

Asked by: Cow147
Thank you for your question. We have validated that it is human reactive, which is why we only recommend it for that use. This antibody has not been tested for mouse use, so we can not say whether it will pick up mouse or not. There is definitely a possibility that it picks up mouse, but we have not tested it. We only warranty it for human reactivity, not that it will not detect any other species.
Answered by: Tech Service
Date published: 2019-01-07
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Rated 3 out of 5 by from Good Tested on cell lysate by Western Blot (1:100), the band is visible, but there are some other bands at higher molecular weight.
Date published: 2019-05-08
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