p27 Antibody (SX53G8.5)recommended for detection of p27 of mouse, rat and human origin by WB, IP, IF and IHC(P)

p27 Antibody (SX53G8.5): sc-53871

p27 Antibody (SX53G8.5) is rated 4.6 out of 5 by 5.
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Datasheets

    • p27 Antibody (SX53G8.5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against purified GST-p27 fusion protein of human origin
    • recommended for detection of p27 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See p27 (F-8): sc-1641 for p27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using p27 (SX53G8.5): sc-53871 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong bands observed in western blot analysis Strong bands observed in western blot analysis of p27 expression in non-transfected and human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-27
Rated 4 out of 5 by from WB: positive results with mouse p27 transfected WB: positive results with mouse p27 transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-04-17
Rated 4 out of 5 by from Robust band in WB using human p27 transfected Robust band in WB using human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Worked well for WB for our p27 studies human Worked well for WB for our p27 studies human thyroid cell lines derived from anaplastic carcinoma(CAL-62, 8305C), papillary carcinoma (B-CPAP, BHT-101,ONCO-DG1), follicular carcinoma (FTC-133, CGTH-W1),differentiated carcinoma (K1). -SCBT Publication Review
Date published: 2015-03-21
Rated 5 out of 5 by from WB: works well with Kip16 cells WB: works well with Kip16 cells, PE25 cells, and HepG2 Liver cancer cells. -SCBT Publication Review
Date published: 2014-02-17
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