p27 Antibody (SPM348)recommended for detection of p27 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and FCM

p27 Antibody (SPM348): sc-56454

p27 Antibody (SPM348) is rated 4.2 out of 5 by 5.
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Datasheets

    • p27 Antibody (SPM348) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant p27 protein of mouse origin
    • recommended for detection of p27 of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See p27 (F-8): sc-1641 for p27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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EPITOPE?

Asked by: Soler
Thank you for your question. This antibody was raised against recombinant p27 of mouse origin. The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2019-01-23

Are there any issues with using sc-56454: p27 (SPM348) mouse monoclonal primary antibody on mouse samples?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56454 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Used for WB at 1:100 dilution on human breast Used for WB at 1:100 dilution on human breast cancer tissue lysates.
Date published: 2015-05-07
Rated 4 out of 5 by from Published immunohistochemical analysis; paraffin Published immunohistochemical analysis; paraffin embedded canine non-neoplastic mammary glands,Adenoma, carcinoma, and Intravascular metastatic tumor cell sections. -SCBT Publication Review
Date published: 2015-03-31
Rated 4 out of 5 by from WB: strong bands in human p27 transfected WB: strong bands in human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-03-31
Rated 4 out of 5 by from Worked well for IHC on paraformaldehyde fixed Worked well for IHC on paraformaldehyde fixed pancreatic tissue sections (obtained from human embryos and fetuses).
Date published: 2015-02-04
Rated 4 out of 5 by from Clean results obtained in western blot analysis Clean results obtained in western blot analysis of p27 expression in Jurkat, HeLa and MCF7 whole cell lysates. -SCBT QC
Date published: 2013-06-26
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