p27 Antibody (0.N.491)recommended for detection of p27 of mouse, rat and human origin by WB, IP, IF and IHC(P)

p27 Antibody (0.N.491): sc-71813

p27 Antibody (0.N.491) is rated 4.6 out of 5 by 11.
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Datasheets

    • p27 Antibody (0.N.491) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length p27 of mouse origin
    • recommended for detection of p27 of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with other mitotic inhibitors
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See p27 (F-8): sc-1641 for p27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can p27 (0.N.491): sc-71813 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, p27 (0.N.491): sc-71813 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Good for detecting mouse and human p27 by WB Good for detecting p27 in mouse (MEF) and human (U2OS) cells at 1:500 dilution.
Date published: 2018-11-14
Rated 5 out of 5 by from It is useful for Western blot! I tested this antibody for TPA or DMBA experiments. While the cells occur apoptosis, this antibody worked, showed its activation.
Date published: 2018-07-05
Rated 5 out of 5 by from Great antibody! worked in my hand. A single strong band was observed using this antibody in various WCLs by western. The signals are always strong and clear.
Date published: 2017-09-11
Rated 5 out of 5 by from good job I used it at a dilution of 1:1000 for WB incubated in 4℃ overnight, and the results were quite good! Clean band with little background for WB in mcf7 and 293t whole cell lysates!
Date published: 2016-12-30
Rated 5 out of 5 by from Excellent antibodiy A good antibody!!!This antibody works very well in western blot detection in the H1299,hela,u2os cells.
Date published: 2016-12-27
Rated 5 out of 5 by from Western blot of this antibody i use this antibody to select high expression p27 celline,at last i got the different exprsssion levels band,very useful!
Date published: 2016-12-13
Rated 5 out of 5 by from strong signal! I used it at a dilution of 1:500 for WB, and the results were great!Clean band with little background for WB in h1299, sw620, hela, u2os whole cell lysates!!
Date published: 2016-12-07
Rated 4 out of 5 by from Clean Clean, singular band observed in western blot analysis of Jurkat and human p27 transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-04-25
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