Anti-p21 Waf1/Cip1/CDKN1A Antibody (SX118) recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP and IF

Anti-p21 Waf1/Cip1/CDKN1A Antibody (SX118): sc-53870

Anti-p21 Waf1/Cip1/CDKN1A Antibody (SX118) is rated 4.9 out of 5 by 10.
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Datasheets

    • p21 Waf1/Cip1/CDKN1A Antibody (SX118) is a mouse monoclonal IgG1 κ, cited in 55 publications, provided at 200 µg/ml
    • raised against amino acids 146-164 of p21 Waf1/Cip1 of human origin
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (SX118) is recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP and IF
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (SX118) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p21 Waf1/Cip1/CDKN1A (SX118): sc-53870.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p21 Waf1/Cip1/CDKN1A Antibody (SX118) for WB applications. These reagents are now offered in bundles with p21 Waf1/Cip1/CDKN1A Antibody (SX118) (see ordering information below).
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What secondary is best to use with p21 (SX118): sc-53870 monoclonal antibody?

Asked by: cjMara
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with p21 (SX118): sc-53870 mouse monoclonal IgG1, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-20
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Rated 5 out of 5 by from Sharp, clean bands! Cleanest, clearest signal out of all the p21 Abs I have used for WB in human cancer cell lines. No nonspecific bands at all.
Date published: 2019-11-27
Rated 4 out of 5 by from good Used in TBST-1X + 5% milk. Dilution 1:1000. There's just one nonspecific signal.
Date published: 2018-04-10
Rated 5 out of 5 by from great! Used at 1:200 for mouse tissue lysates. Antibody gave good clear band! Recommend!
Date published: 2018-03-23
Rated 5 out of 5 by from Great antibody We tried 3-4 antibodies for p21 and this antibody worked the best for our Rat cell line.
Date published: 2018-03-15
Rated 5 out of 5 by from Quite good antibody! 1:500 diluted for wb of rat protein,the band is strong and special!
Date published: 2017-12-30
Rated 5 out of 5 by from Excellent antibody for p21! Strong and reliable, this antibody gives great Western blot results. Bands are strong and specific.
Date published: 2017-01-09
Rated 5 out of 5 by from Great antibody Great antibody! Very good results in WB.
Date published: 2015-06-15
Rated 5 out of 5 by from Great WB data obtained using untreated and Great WB data obtained using untreated and Sodium Phenylbutyrate treated Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-03-30
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