Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-8) recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-8): sc-271610

Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-8) is rated 4.5 out of 5 by 13.
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Datasheets

    • p21 Waf1/Cip1/CDKN1A Antibody (F-8) is a mouse monoclonal IgG2a κ, cited in 31 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 124-164 at the C-terminus of p21 Waf1/Cip1 of human origin
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-8) is recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p21 Waf1/Cip1/CDKN1A (F-8): sc-271610.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p21 Waf1/Cip1/CDKN1A Antibody (F-8) for WB applications. These reagents are now offered in bundles with p21 Waf1/Cip1/CDKN1A Antibody (F-8) (see ordering information below).
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What is the best secondary to use with your mouse monoclonal antibodies for WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 5 out of 5 by from Worked excellent on our western blots We used it 1:1000 on our immunoblots. The signals were excellent.
Date published: 2021-07-29
Rated 1 out of 5 by from very bad product it is not working in cell lysate especially human cell lines (hepG2)
Date published: 2020-10-22
Rated 5 out of 5 by from Great antibody! Used at 1:200 for mouse tissue lysates. Antibody gave good clear band! Recommend!
Date published: 2018-03-23
Rated 5 out of 5 by from very strong signal Specific WB band in HeLa whole cell lysate, 1:300 dilution, Good data!!!
Date published: 2017-04-05
Rated 5 out of 5 by from Strong and Specific p21 (F-8) gives a strong specific band for Western blot (WB) at 21 kDa.
Date published: 2016-12-20
Rated 5 out of 5 by from great 1:1000 dilution for detection of p21 in DLBCL lymphoma cells, incubate with the filter overnight at 4C. It's very sensitive and stable with the clean background.
Date published: 2016-12-17
Rated 4 out of 5 by from This has worked well in WB with mouse liver This has worked well in WB with mouse liver.
Date published: 2015-12-07
Rated 5 out of 5 by from This has worked well in WB with mouse liver This has worked well in WB with mouse liver
Date published: 2015-10-16
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