Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM

Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5): sc-6246

Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) is rated 4.4 out of 5 by 91.
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Datasheets

    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) is a mouse monoclonal IgG2b κ p21 Waf1/Cip1/CDKN1A antibody, cited in 1,596 publications, provided at 200 µg/ml
    • raised against amino acids 1-159 representing full length p21 Waf1/Cip1 of mouse origin
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) is recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p21 Waf1/Cip1/CDKN1A (F-5): sc-6246.
    • m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for p21 Waf1/Cip1/CDKN1A Antibody (F-5) for WB and IHC(P) applications. These reagents are now offered in bundles with p21 Waf1/Cip1/CDKN1A Antibody (F-5) (see ordering information below).
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What is the recommended antigen retrieval protocol for IHC-P using this antibody?

Asked by: nmurao
Thank you for your question. You can find our recommended antigen retrieval method in our standard protocol which is available under this link: https://www.scbt.com/de/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2020-09-28

I'm having issues with the detection of p21 wih this antibody in A549 cells treated with nutlin or etoposide. Can you recommend western blot conditions for this cell line and protein? I'm using 1:200 dilution in TBST milk. Thanks

Asked by: eabd2
Thank you for your question. We are sorry to hear that this antibody isn't performing as expected. We want you to be successful in your research! Please contact our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody.
Answered by: Tech Service
Date published: 2019-11-14

What is the recommended blocking solution for p21 ab in WB?

Asked by: Sonia S
Tack för din fråga! We recommend blocking in 5% milk and TBS-T for Western blot with our p21 antibodies.
Answered by: Tech Service
Date published: 2021-02-04

I used this p21 antibody for Western blotting of both cell sample and tissue sample. For cell sample, the band is at 21 kD; but for tissue, the band is at around 25 kD. Is that normal? What is the probable explanation of it?

Asked by: Liuershiba
Thank you for your question. The difference in observed molecular weight in your samples could be due to post-translational modification of the p21 protein in the tissue that is not present in the cells. However, inclusion of proper positive and negative controls is always recommended.
Answered by: Technical Support
Date published: 2019-06-16

If I am using this antibody to stain P21 positive cells in the brain via immunofluorescence, what secondary antibody should I use?

Asked by: khanson
Thank you for your question. p21 Antibody (F-5) is a mouse monoclonal IgG2b, so we would recommend using a secondary antibody that is anti-mouse IgG, or anti-mouse IgG2b for detection. One example for immunofluorescence is our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 488 (CFL 488), m-IgGκ BP-CFL 488: sc-516176.
Answered by: Technical Support
Date published: 2019-04-20

p21 Antibody (F-5): sc-6246  Is this antibody work for Rhesus monkey?

Asked by: Fei38750
Thank you for your question. We are aware of several product citations using this antibody in monkey, including Vero cells. However, nothing specifically in Rhesus macaque.
Answered by: Technical Support
Date published: 2019-04-13

I've recently got the sample F-15 antibody sample and ran the WB using C2C12, mouse myoblast cells. But all the time, I can see the major bands between 15 and 20kD. I used 15% gel. Is it correct size? The size of this protein is not 21kDa?

Asked by: Aiden
Thank you for your question. As you mentioned the expected molecular weight of p21 is 21 kDa, and that is what is expected when using this mouse monoclonal, sc-6246, p21 (F-5). We would recommend including a secondary antibody control for your Western blot, where you incubate the membrane without primary antibody. That would identify any non-specific bands that are the result of your secondary antibody alone. A band observed below 15 kDa would be a concern, but since it is normal to see a variation of +/- 5Kda in a western (could be marker differences, gel uneveness, etc), I believe the band you are seeing is p21. Please contact our Technical Service Department if you would like to discuss this further!
Answered by: Tech Service
Date published: 2021-06-03

Is it possible to provide the MSDS fiche for this product?

Asked by: BDCC
Thank you for your question. We do not provide MSDSs for our antibody products, but please contact our European office and they will be glad to help get you the information you need. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-09
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Rated 4 out of 5 by from Works well! We blotted several times with this antibody and it works perfectly!
Date published: 2021-06-16
Rated 5 out of 5 by from 功能很出色! 我一个月前购买了此产品,并为此感到高兴,下次有机会还会来购买!另外圣克鲁兹的产品价格便宜实惠,实验效果好,实在是太棒了!
Date published: 2020-11-20
Rated 3 out of 5 by from è cross reattivo nel cane ma non nel cavallo non è cross reattivo nella specie equina, è comunque cross reattivo nel cane
Date published: 2020-10-07
Rated 3 out of 5 by from For WB We got a free sample of this antibody for WB. This antibody could detect p21 of p21 transfected 293T cell lysate easily. We will use this antibody for WB from now on.
Date published: 2019-07-17
Rated 2 out of 5 by from Immunofluorescence on mouse endothelial cells I tested the antibody in immunofluorescence and I obtained nuclear staining of only few cells.
Date published: 2019-04-28
Rated 5 out of 5 by from Good Ab for IF This antibody is good for immunofluorescent staining (1:50 in 1%BSA in PBS). Highly recommended!
Date published: 2018-11-13
Rated 5 out of 5 by from Very good anti-p21 in western blot Very good anti-p21 in western blot at 1/1000 dilution in TBS-tween 5% milk
Date published: 2018-10-03
Rated 5 out of 5 by from good product Good antibody with clean background for Western Blot.
Date published: 2018-09-11
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