Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) is a mouse monoclonal IgG2b κ p21 Waf1/Cip1/CDKN1A antibody, cited in 1,596 publications, provided at 200 µg/ml
raised against amino acids 1-159 representing full length p21 Waf1/Cip1 of mouse origin
Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) is recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
Anti-p21 Waf1/Cip1/CDKN1A Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p21 Waf1/Cip1/CDKN1A (F-5): sc-6246.
m-IgG Fc BP-HRP and m-IgG2b BP-HRP are the preferred secondary detection reagents for p21 Waf1/Cip1/CDKN1A Antibody (F-5) for WB and IHC(P) applications. These reagents are now offered in bundles with p21 Waf1/Cip1/CDKN1A Antibody (F-5) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
p21 Waf1/Cip1 Antibody (F-5) is a high quality monoclonal p21 Waf1/Cip1 antibody (also designated CIP1 antibody, WAF1 antibody, CDKN1A antibody or p21 antibody) suitable for the detection of the p21 Waf1/Cip1 protein of mouse, rat and human origin. p21 Waf1/Cip1 Antibody (F-5) is available as both the non-conjugated anti-p21 Waf1/Cip1 antibody form, as well as multiple conjugated forms of anti-p21 Waf1/Cip1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. It is now well established that cyclins play a positive role in promoting cell cycle transitions via their ability to associate with and activate their cognate cyclin-dependent kinases (Cdks). Cdk2 associates with cyclins A, D and E, and has been implicated in the control of the G1 to S phase transition in mammals. p21 Waf1/Cip1, also designated Cip1 or WAF1, has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. The novel Cdk-interacting protein, p21 Waf1/Cip1 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 Waf1/Cip1 protein, which is identified using the p21 Waf1/Cip1 Antibody (F-5) in various research applications, is inducible by wildtype, but not mutant, p53. The mouse homolog of p21 Waf1/Cip1 is designated CAP20.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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