Anti-p21 Waf1/Cip1/CDKN1A Antibody (DCS-60) recommended for detection of p21 Waf1/Cip1 of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-p21 Waf1/Cip1/CDKN1A Antibody (DCS-60): sc-56335

Anti-p21 Waf1/Cip1/CDKN1A Antibody (DCS-60) is rated 4.0 out of 5 by 3.
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Datasheets

    • p21 Waf1/Cip1/CDKN1A Antibody (DCS-60) is a mouse monoclonal IgG2a κ, cited in 24 publications, provided at 200 µg/ml
    • raised against full length p21 Waf1/Cip1 of human origin
    • recommended for detection of p21 Waf1/Cip1 of human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with other mitotic inhibitors
    • See p21 Waf1/Cip1/CDKN1A (F-5): sc-6246 for p21 Waf1/Cip1/CDKN1A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p21 Waf1/Cip1/CDKN1A Antibody (DCS-60). This work is in progress.
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What blocking solution should I use with p21 (DCS-60): sc-56335 monoclonal antibody for Western blot?

Asked by: Germaine
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-12
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Rated 4 out of 5 by from The antibody works well for Western blotting The antibody works well for Western blotting detecting endogenous p21 in C32 whole cell lysate. -SCBT QC
Date published: 2015-03-26
Rated 4 out of 5 by from Great antibody for Western Great antibody for Western, strong p21 band detected in HeLa nuclear extract. -SCBT QC
Date published: 2015-01-23
Rated 4 out of 5 by from Good specificity and signal strength for Good specificity and signal strength for WB in Raji whole cell lysate. -SCBT QC
Date published: 2013-01-29
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