Anti-p21 Waf1/Cip1/CDKN1A Antibody (B-2) recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-p21 Waf1/Cip1/CDKN1A Antibody (B-2): sc-271532

Anti-p21 Waf1/Cip1/CDKN1A Antibody (B-2) is rated 4.7 out of 5 by 6.
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Datasheets

    • p21 Waf1/Cip1/CDKN1A Antibody (B-2) is a mouse monoclonal IgG1 κ, cited in 30 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 124-164 at the C-terminus of p21 Waf1/Cip1 of human origin
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (B-2) is recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p21 Waf1/Cip1/CDKN1A (B-2): sc-271532.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p21 Waf1/Cip1/CDKN1A Antibody (B-2). This work is in progress.
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What is the recommended secondary reagent to use with p21 (B-2): sc-271532 monoclonal antibody for Western blot?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. This primary antibody has a kappa light chain, so for Western blotting we would recommend m-IgGκ BP-HRP: sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 4 out of 5 by from Good for detecting mouse and human p21 by WB Good for detecting p21 in mouse (MEF) and human (U2OS) cells at 1:500 dilution.
Date published: 2018-11-14
Rated 5 out of 5 by from Great for WB! Used at 1:200 for mouse tissue lysates. Antibody gave good clear band! Recommend!
Date published: 2018-03-23
Rated 4 out of 5 by from Good Clear band in WB analysis of p21 expression in Rat VSMC.
Date published: 2017-11-24
Rated 5 out of 5 by from Strong WB Very clear western blot data; strong signal, low background
Date published: 2017-02-05
Rated 5 out of 5 by from Good alternative to sc-397 The (B-2) clone is a good alternative to the now discontinued rabbit polyclonal, sc-397: p21 (C-19) antibody. This clone offers greater specificity and no lot to lot variation.
Date published: 2016-12-29
Rated 5 out of 5 by from Work well with mouse p21 transfected 293T Work well with mouse p21 transfected 293T whole cell lysates by WB. -SCBT QC
Date published: 2014-08-19
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