Anti-p21 Waf1/Cip1/CDKN1A Antibody (187) recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-p21 Waf1/Cip1/CDKN1A Antibody (187): sc-817

Anti-p21 Waf1/Cip1/CDKN1A Antibody (187) is rated 4.6 out of 5 by 16.
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Datasheets

    • p21 Waf1/Cip1/CDKN1A Antibody (187) is a mouse monoclonal IgG1 κ, cited in 366 publications, provided at 200 µg/ml
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (187) is recommended for detection of p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p21 Waf1/Cip1/CDKN1A Antibody (187) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-817 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p21 Waf1/Cip1/CDKN1A (187): sc-817.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p21 Waf1/Cip1/CDKN1A Antibody (187) for WB and IHC(P) applications. These reagents are now offered in bundles with p21 Waf1/Cip1/CDKN1A Antibody (187) (see ordering information below).
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Can this be used for Co-IP in mouse cancer cell lines? I have not been able to detect a band for p21 after pull down. If not, any alternative?

Asked by: app22
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-01-28

What is the recommended secondary reagent to use with this mouse monoclonal primary antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from Good for immunofluorescence and WB The antibody works very well in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA. Moreover the sample works good at the ratio of 1:500 in WB in the whole cell lisate.
Date published: 2020-11-30
Rated 5 out of 5 by from good product It is a good antibody for Western Blot experiment with clean background.
Date published: 2018-09-11
Rated 5 out of 5 by from Great! I got the sample of this antibody to test the senescence process in the muscle of my mice model. Comparing with the non-detectable level of p21 in WT control mice, I got significant strong signal in my severe disease mice model. The antibody works great. Thanks.
Date published: 2018-09-10
Rated 5 out of 5 by from Great antibody! Works great at 1:200 in mouse tissue samples. Highly recommend!
Date published: 2018-03-22
Rated 4 out of 5 by from Good antibody Works well for Western Blot..the dilution that i used is 1:200
Date published: 2017-12-21
Rated 3 out of 5 by from NOT good This antibody is NOT good for western blot. (used at 1:1000 dilution)
Date published: 2017-09-29
Rated 5 out of 5 by from Great antibody Worked great. Strong, clean band in HCT116 cells stimulated with various p53 activators. Worked equally well as clone F-5.
Date published: 2017-08-15
Rated 5 out of 5 by from Great antibody Very nice wb and ihc data although not quite as stong as the f-5 clone also available from scbt
Date published: 2017-01-03
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