Anti-p21 Waf1/Cip1/CDKN1A Antibody (0.N.488) recommended for detection of p21 Waf1/Cip1 of human origin by WB, IP, IF and IHC(P)

Anti-p21 Waf1/Cip1/CDKN1A Antibody (0.N.488): sc-71811

Anti-p21 Waf1/Cip1/CDKN1A Antibody (0.N.488) is rated 5.0 out of 5 by 3.
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Datasheets

    • p21 Waf1/Cip1/CDKN1A Antibody (0.N.488) is a mouse monoclonal IgG2a κ, cited in 15 publications, provided at 200 µg/ml
    • raised against full length p21 Waf1/Cip1 of human origin
    • recommended for detection of p21 Waf1/Cip1 of human origin by WB, IP, IF and IHC(P); non cross-reactive with other mitotic inhibitors
    • See p21 Waf1/Cip1/CDKN1A (F-5): sc-6246 for p21 Waf1/Cip1/CDKN1A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for p21 Waf1/Cip1/CDKN1A Antibody (0.N.488) for WB and IHC(P) applications. These reagents are now offered in bundles with p21 Waf1/Cip1/CDKN1A Antibody (0.N.488) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-71811: p21 (0.N.488) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from great I used this antibody to detect the expression of P21 in human macrophages and liver cells to analyze the influence on cell cycle.
Date published: 2016-12-05
Rated 5 out of 5 by from Works well for WB detection in HeLa nuclear Works well for WB detection in HeLa nuclear extract. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Good signal strength for WB in C32 whole Good signal strength for WB in C32 whole cell lysate. -SCBT QC
Date published: 2014-08-19
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