p-Tyr Antibody (PY99)recommended for detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P)

p-Tyr Antibody (PY99): sc-7020

p-Tyr Antibody (PY99) is rated 4.7 out of 5 by 29.
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Datasheets

    • p-Tyr Antibody (PY99) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against p-Tyr
    • p-Tyr Antibody (PY99) is recommended for detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P); non cross-reactive with phosphoserine or phosphothreonine
    • p-Tyr Antibody (PY99) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • available conjugated to biotin for WB, IHC(P) and ELISA; and to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Tyr (PY99): sc-7020.
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When I use this antibody for immunohistochemistry. Is antigen retrieval necessary?

Asked by: AH2019
Thank you for your question. Yes, we recommend performing an antigen retrieval step if you are doing IHC on formalin fixed paraffin embedded tissue sections.
Answered by: Tech Service
Date published: 2019-10-11

Can I use 10% milk to block when using this antibody? I am interested in getting it conjugated with the alexa 790 to be used with LI-COR®Odyssey®.

Asked by: nsal
Thank you for your question. We recommend blocking in 5% milk with TBS-T, and it should be fine with the LiCOR Odyssey.
Answered by: Tech Service
Date published: 2019-09-24

Is there any species specificity for this antibody? Or is there any cross-reactivity?

Asked by: beni
Thank you for your question. This antibody has broad species reactivity and is specific for phosphotyrosine-containing proteins; non cross-reactive with phosphoserine or phosphothreonine.
Answered by: Technical Support
Date published: 2019-09-11

I want to measure insulin sensitivity and several tyrosine domains are phosphorylated in response to insulin. For example in both insulin receptors and in IRS. Does the antibody detetct all p-tyr, and I differentiate between them depending on weight?

Asked by: Ahmed_hassan
Thank you for your question. Yes, you are correct, this mouse monoclonal antibody, p-Tyr (PY99): sc-7020, specifically detects phosphotyrosine-containing proteins (and does not cross-reactive with phosphoserine or phosphothreonine-containing proteins). If you are using the antibody for Western blotting, then the molecular weight you detect in your samples is representative of different phosphorylated proteins. You would need to do further testing to identify which specific proteins are phosphorylated in your samples.
Answered by: Technical Support
Date published: 2017-10-09

What secondary reagent should I use with p-Tyr Antibody (PY99) for Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-15

Is it possible to use this p-Tyr (PY99) mouse monoclonal antibody for immunohistochemistry in mouse tissue?

Asked by: Trav11
Yes, it is possible. If working with mouse tissue, we recommend using our directly conjugated mouse monoclonal primary antibodies. The p-Tyr Antibody (PY99): sc-7020 is currently available conjugated to phycoerythrin (sc-7020 PE), fluorescein (sc-7020 FITC), Alexa Fluor® 488 (sc-7020 AF488), Alexa Fluor® 647 (sc-7020 AF647), Alexa Fluor® 405 (sc-7020 AF405), biotin (sc-7020 B) or alkaline phosphatase (sc-7020 AP).
Answered by: Tech Service 8
Date published: 2019-11-07
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Rated 4 out of 5 by from good pTr antibody Works well to detect tyrosine phosphorylation in whole cell or tissue lysates.
Date published: 2018-05-17
Rated 5 out of 5 by from good for WB I use 1:1000 in 5%BSA, it is less specific for mouse lung tissue, I haven't try primary cell but I hope it works.
Date published: 2018-03-12
Rated 4 out of 5 by from Good for WB I tried a 1/500 dilution in 3% BSA and it worked well. Good signal.
Date published: 2017-10-24
Rated 5 out of 5 by from IHC detection IHC detected the expression of P-Tyr in gastric carcinoma tissues ,it gave cytoplasmic staining ,good signal for us.
Date published: 2017-09-16
Rated 5 out of 5 by from staining IHC(1/100) staining in human skin tissu, very sensitive
Date published: 2017-09-08
Rated 5 out of 5 by from very good Gives clear for nuclear extracts in western blot; had a strong signal in the western blot.
Date published: 2017-08-17
Rated 5 out of 5 by from successful experiment Very good for WB with a dilution of 1:1000, good signal obtained in WB.
Date published: 2017-06-01
Rated 5 out of 5 by from WB and IP we are successful. Very strong signal at a 1:1000 dilution in western blot, and also for IP's. Worked great on cortical cultures.
Date published: 2017-06-01
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