Anti-p-Tyr Antibody (PY20) recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM

Anti-p-Tyr Antibody (PY20): sc-508

Anti-p-Tyr Antibody (PY20) is rated 4.8 out of 5 by 19.
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_en_US, sid_508, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 7ms
  • REVIEWS, PRODUCT
loading
5
16
4
3
3
0
2
0
1
0

Datasheets

    • p-Tyr Antibody (PY20) is a mouse monoclonal IgG2b κ, cited in 1,305 publications, provided at 200 µg/ml
    • raised against p-Tyr
    • p-Tyr Antibody (PY20) is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM
    • Anti-p-Tyr Antibody (PY20) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Tyr (PY20): sc-508.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Tyr Antibody (PY20) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Tyr Antibody (PY20) (see ordering information below).
loading

Hello! Please tell me if p-Tyr Antibody (PY20): sc-508 is working in ELISA assay. If yes, could you recommend me a starting dilution range! I want to know if the p-Tyr Antibody (PY20) HRP variant is recommended for ELISA and in what dilution.

Asked by: Rodica
Thank you for your question. p-Tyr Antibody (PY20): sc-508 is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM. It is also available conjugated to HRP for ELISA. The starting dilution is 1:30, with a dilution range of 1:30-1:3000.
Answered by: Tech Support Europe
Date published: 2020-11-27

Which phosphotyrosine antibody is more sensitive in Western Blot: p-Tyr (PY99) or p-Tyr (PY20)? Which concentration is the recommended?

Asked by: AngelesMZ
Thank you for your question. Both p-Tyr Antibody (PY20): sc-508 and p-Tyr Antibody (PY99): sc-7020 are featured in several hundred publications and are recommended for western blot and other applications. Both antibodies have a recommended starting dilution of 1:200 and work in a dilution range 1:100-1:1000. Either antibody is perfectly suited for western blotting, so you could order one or the other or get in touch with our technical service to arrange for free samples of the two antibodies.
Answered by: Technical Support Europe
Date published: 2019-04-12

for blocking, what would you recommend? Many people recommend 5% of BSA for phospho-protein..( should I stick with this rule.). but some phospho-protein can worked well with.. Skim milk (depends the antibody protocol..)

Asked by: blessing
Thank you for your question. We recommend using 1% milk, 1% BSA in 0.05% Tween-20 for blocking and antibody incubations.
Answered by: Technical Support
Date published: 2019-02-01

EGF-treated とは、具体的にどのようにするものでしょうか。ご教授お願い致します。

Asked by: k 2018
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-21

Can I use p-Tyr Antibody (PY20): sc-508 for immunohistochemical staining of mouse tissue

Asked by: Anonymous
We recommend using a directly conjugated mouse monoclonal primary antibody. See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/p-tyr-antibody-py99
Answered by: Tech Service 6
Date published: 2016-12-16

What is the recommended secondary detection reagent in Western blot?

Asked by: Anonymous
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-16
  • y_2021, m_10, d_19, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_en_US, sid_508, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 8ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Always reliable! I have relied on you for over 20 years and you have always delivered an excellent product on time. Your phone staff is friendly and helpful.
Date published: 2018-03-08
Rated 4 out of 5 by from Very good I have used Py20 Antibody from Santa and it worked great.
Date published: 2017-12-14
Rated 4 out of 5 by from As expected Gives WB bands as expected at 1:500 dilution in 5% SA (block in non-fat dry milk).
Date published: 2017-12-02
Rated 5 out of 5 by from Very Strong Signal I compare different products for art antibody found p-Tyr antibody is the most sensitive, sc-508 is the most reliable quality.
Date published: 2017-10-28
Rated 5 out of 5 by from Excellent antibody Strong signal and low background on immunoprecipitated protein.
Date published: 2017-09-01
Rated 5 out of 5 by from good result antibody It is an excellent antibody. gives a single band in my hand.
Date published: 2017-05-27
Rated 5 out of 5 by from antibody for WB I use the dilution 1:800, get very clean bands.I like
Date published: 2017-05-07
Rated 5 out of 5 by from High Quality Great antibody for detection of any protein with a phosphorylated Tyrosine, can be used in a number of applications, not species specific.
Date published: 2016-12-20
  • y_2021, m_10, d_19, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.19
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_19
  • loc_en_US, sid_508, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT