Anti-p-Tyr Antibody (2C8) recommended for detection of phosphotyrosine-containing proteins of and origin by WB and IP

Anti-p-Tyr Antibody (2C8): sc-81529

Anti-p-Tyr Antibody (2C8) is rated 4.7 out of 5 by 3.
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Datasheets

    • p-Tyr Antibody (2C8) is a mouse monoclonal IgG1, cited in 7 publications, provided at 50 µg/0.5 ml
    • raised against a synthetic tyrosine-phosphorylated peptide of human origin
    • recommended for detection of phosphotyrosine-containing proteins of and origin by WB and IP
    • See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Tyr (2C8): sc-81529.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Tyr Antibody (2C8). This work is in progress.
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I am using p-Tyr (2C8): sc-81529 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from recommended I got specific band at 1:1000 dilution, the result is out of my expect
Date published: 2017-05-10
Rated 5 out of 5 by from Produced publishable WB data in human This p-Tyr monoclonal antibody was referenced in a recent publication for the use in WB on human neuroblastoma
Date published: 2017-02-01
Rated 4 out of 5 by from Publishable WB data obtained using Wistar Publishable WB data obtained using Wistar rat liver samples. -SCBT Publication Review
Date published: 2013-08-07
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