Anti-p-Thr Antibody (H-2) recommended for detection of phosphothreonine-containing proteins of broad origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-p-Thr Antibody (H-2): sc-5267

Anti-p-Thr Antibody (H-2) is rated 4.3 out of 5 by 15.
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Datasheets

    • p-Thr Antibody (H-2) is a mouse monoclonal IgG1 κ, cited in 83 publications, provided at 200 µg/ml
    • raised against p-Thr
    • p-Thr Antibody (H-2) is recommended for detection of phosphothreonine-containing proteins of broad origin by WB, IP, IF, IHC(P), FCM and ELISA; non cross-reactive with phosphotyrosine or phosphoserine
    • Anti-p-Thr Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • available conjugated to biotin for WB, IHC(P) and ELISA; and to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Thr (H-2): sc-5267.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p-Thr Antibody (H-2) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Thr Antibody (H-2) (see ordering information below).
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Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-5267 PE), fluorescein (sc-5267 FITC), Alexa Fluor® 488 (sc-5267 AF488) or Alexa Fluor® 647 (sc-5267 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2017-01-04
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Rated 1 out of 5 by from Did not work for co-IP in our hands Despite of several good reviews, this antibody did not work for co-IP in our hands.
Date published: 2018-06-11
Rated 4 out of 5 by from Work in 293T cells expressing targeted protein Work well with my protein overexpressing in 293T cells.
Date published: 2017-12-07
Rated 3 out of 5 by from Good results but fainted bands I have tried this antibody in human cells starved for glucose. I loaded 20ug of proteins and used a 1/1000 dilution and incubation OVN. The profiles were good but the signal intensity was pretty low.
Date published: 2017-10-27
Rated 3 out of 5 by from Not working in WB I use the mouse NIH3T3-L1 cells and western blot with whole cell lysates. It does not work.
Date published: 2017-08-22
Rated 5 out of 5 by from Great product The p-Thr shows strong pattern for immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.
Date published: 2017-08-15
Rated 4 out of 5 by from Good Antibody We used this Ab to probe p-Thr in our IP samples and the data are great!
Date published: 2017-05-17
Rated 5 out of 5 by from Versitle Antibody Nice and clean data produced by Western blot, IHC (P), IF and Flow Cytometry! Cited in over 40 publications! Highly recommended!
Date published: 2017-01-10
Rated 5 out of 5 by from Outstanding Vert stong wb,if and ihc results;best available p-Thr antibody
Date published: 2016-12-31
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