Anti-p-Tau Antibody (PHF-6) recommended for detection of Tau phosphorylated at Thr 231 of human origin by WB, IP, IF and IHC(P)

Anti-p-Tau Antibody (PHF-6): sc-32276

Anti-p-Tau Antibody (PHF-6) is rated 4.3 out of 5 by 3.
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Datasheets

    • p-Tau Antibody (PHF-6) is a mouse monoclonal IgG1 κ, cited in 7 publications, provided at 200 µg/ml
    • raised against highly purified PHF-Tau preparation of human origin
    • recommended for detection of Tau phosphorylated at Thr 231 of human origin by WB, IP, IF and IHC(P)
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Tau Antibody (PHF-6) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Tau Antibody (PHF-6) (see ordering information below).
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I am using p-Tau (PHF-6): sc-32276 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good antibody for WB I've used this ab for wb and the result is good. I would like to recommed this one for further use.
Date published: 2017-03-09
Rated 5 out of 5 by from Great for IHC(P) The p-Tau Antibody (PHF-6): sc-32276 provides excellent results when performing IHC with formalin fixed, paraffin-embedded tissue sections.
Date published: 2017-01-20
Rated 4 out of 5 by from Positive results in WB using paired helical Positive results in WB using paired helical filaments from human brain. -SCBT QC
Date published: 2013-07-20
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