Anti-p-Ser/Phosphoserine Antibody (4A9) detects a broad range of serine-phosphorylated proteins in crude cell extracts, preferring + charged AAs directly neighbored to phosphoserine of mouse, rat, human and canine origin by WB and IP

Anti-p-Ser/Phosphoserine Antibody (4A9): sc-81517

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Anti-p-Ser/Phosphoserine Antibody (4A9) is rated 4.0 out of 5 by 1.
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Datasheets

    • p-Ser/Phosphoserine Antibody (4A9) is a mouse monoclonal IgM, cited in 1 publications, provided at 50 µg/0.5 ml
    • raised against phosphoserine
    • recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine of mouse, rat, human and canine origin by WB and IP
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Ser/Phosphoserine Antibody (4A9). This work is in progress.
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I am using p-Ser (4A9): sc-81517 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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