Anti-p-Ser/Phosphoserine Antibody (4A3) recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts...of mouse, rat, human and canine origin by WB and IP

Anti-p-Ser/Phosphoserine Antibody (4A3): sc-81516

Anti-p-Ser/Phosphoserine Antibody (4A3) is rated 3.8 out of 5 by 9.
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Datasheets

    • p-Ser/Phosphoserine Antibody (4A3) is a mouse monoclonal IgM κ, cited in 8 publications, provided at 50 µg/0.5 ml
    • raised against phosphoserine
    • recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine of mouse, rat, human and canine origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Ser/Phosphoserine (4A3): sc-81516.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Ser/Phosphoserine Antibody (4A3). This work is in progress.
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What is the recommended storage condition for p-Ser (4A3): sc-81516?

Asked by: jerojero
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2018-06-03
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Rated 3 out of 5 by from worked in WB Phosphorylation was detected in WB using human lysate derived pull-downed protein.
Date published: 2019-07-23
Rated 1 out of 5 by from Not working for WB in our hand We used his antibody for Western blot using human cell nuclear extract. Surprising to us, we did not detect any signal considering so many good reviews from other users.
Date published: 2018-06-05
Rated 5 out of 5 by from Work well in WB. I got this sample, work well in WB after IP. Possibly need optimization in case.
Date published: 2018-03-13
Rated 3 out of 5 by from Better than 16B4 Tested this on Hela lysates. The signals detected were stronger than the 16B4 pSer ab. Background was fine.
Date published: 2018-02-14
Rated 4 out of 5 by from Works for IP samples I used the antibody for post-IP Western blot. I diluted the antibody 1:200 in milk overnight. Saw clear bands. I think 1:500 dilution should also work well.
Date published: 2017-12-01
Rated 4 out of 5 by from Nice antibody We used it last month for IP. After IP with this antibody we probed with many other antibodies. Works well.
Date published: 2017-11-13
Rated 5 out of 5 by from has the high stablity Works well for Western blot at 1:300, this antibody is good.
Date published: 2017-08-15
Rated 5 out of 5 by from Good Antibody for WB Good antibody for detecting p-Ser in WB. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, canine, and human samples.
Date published: 2017-01-26
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