Anti-p-Ser/Phosphoserine Antibody (16B4) recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts...of mouse, rat, human and canine origin by WB and IP

Anti-p-Ser/Phosphoserine Antibody (16B4): sc-81514

Anti-p-Ser/Phosphoserine Antibody (16B4) is rated 3.9 out of 5 by 22.
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Datasheets

    • Anti-p-Ser/Phosphoserine Antibody (16B4) is a mouse monoclonal IgM κ p-Ser/Phosphoserine antibody, cited in 64 publications, provided at 50 µg/0.5 ml
    • raised against phosphoserine
    • recommended for detection of a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine of mouse, rat, human and canine origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Ser/Phosphoserine (16B4): sc-81514.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Ser/Phosphoserine Antibody (16B4). This work is in progress.
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Is there any possibility that p-Ser Antibody (16B4): sc-81514 does work for yeast samples as well...?

Asked by: yolyol
Thank you for your question. This antibody has only been tested in samples of mouse, rat, human and canine origin. It has not been tested in yeast species.
Answered by: Tech Support Europe
Date published: 2021-02-26

Hello, Which buffer is better to detect p-Ser antibody, skim-milk or 3-5% BSA? I immunoprecipitated phospho-protein and used 3% BSA in TBS-T buffer for blocking and 1st antibody but I couldn't get specific phosphorylation band.

Asked by: chohee0720
Thank you for your question. Please contact Technical Service directly for troubleshooting advice on your experiment.
Answered by: Tech Support Europe
Date published: 2021-05-11

HI , I recently purchased this pserine antibody and I do see a band at 20kDa. why do i see a band arounf 20kda for pSerine?

Asked by: Dhanusha
Thank you for your question. As p-Ser Antibody (16B4): sc-81514 detects a broad range of serine-phosphorylated proteins in crude cell extracts, a signal at 20 kDa can respond to a large variety of different proteins of this molecular weight. The identity of this protein could be determined by assays such as MALDI-TOF.
Answered by: Tech Support Europe
Date published: 2020-07-14

The p-Ser Antibody (16B4) antibody is a monoclonal mouse IgM κ (kappa light chain). Still, you recommend the m-IgGκ BP-HRP: sc-516102 as a secondary antibody. But this a IgG-kappa specific ab. Would it still work?

Asked by: PeterPM
Thank you for your question. Yes, m-IgGκ BP-HRP: sc-516102 can detect any antibody that possesses a kappa light chain, including p-Ser Antibody (16B4): sc-81514, other IgM, and IgA antibodies.
Answered by: Technical Support Europe
Date published: 2019-05-06

Just wondering if your products of sc-81516 and sc-81514 recognize any of the phosphorylated S/T/Y, or they require certain residues beside these phosphorylated residues (like a certain motif).

Asked by: Zhyuhao
Thank you for your question. These antibodies both detect a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Additional information regarding the detected residues is not available.
Answered by: Tech Service
Date published: 2019-04-10

"preferring positively charged amino acids directly neighbored to phosphoserine". Does it mean that phosphoserine without neighboring positively charged amino acids will not be recognized by the 16B4 Ab ?

Asked by: Yo14
Thank you for your question. The antibody recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with pSK and pSP motifs strongly, recognizing substrates of MAP/SAP kinases and CDC kinases. Phosphoserine detection using an antibody generally is affected by the surrounding amino acids sequence, but it is possible that it can detect phosphoserine without neighboring + charged amino acids.
Answered by: Tech Service
Date published: 2018-08-09

Hi, is there any possibility to receive a sample from this antibody? Thanks for your answer.

Asked by: Constanze
Thank you for your question. We do not currently offer sample sizes for our p-Ser Antibody (16B4): sc-81514. This is a very popular and well cited antibody. If you purchase the antibody, please know that it is covered by our generous product warranty for one year from the date of purchase. If it does not work in any of the recommended applications, please contact our Technical Service Department and we can provide you with a replacement or refund.
Answered by: Technical Support
Date published: 2017-03-01

What is the expected molecular weight in Western blot when using p-Ser Antibody (16B4): sc-81514?

Asked by: cjMara
Thank you for your question. This antibody is recommended for detection of a broad range of serine-phosphorylated proteins. Therefore, the observed molecular weight is dependent upon which specific phosphorylated protein(s) you are evaluating with this antibody.
Answered by: Technical Support
Date published: 2016-12-22
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Rated 1 out of 5 by from 使えなかった 一切バンドがでなかった。通常リン酸化抗体はブロードなバンドがみえるが、ひとつもバンドがみえなかった。
Date published: 2020-11-18
Rated 3 out of 5 by from Pretty good antibody The antibody works pretty well upon immunoprecipitation of the phosphorylated protein. On total cellular extract is too weak.
Date published: 2020-10-07
Rated 3 out of 5 by from Worked in WB phosphorylation was detected in Pull downed protein in WB
Date published: 2019-07-23
Rated 5 out of 5 by from Successful detection Successful detection of Ser phosphorylation in immunoprecipitates
Date published: 2018-12-07
Rated 3 out of 5 by from P-Ser Antibody Compared to other p-ser abs there was a lower background. Signal was much lower too. Tested in HeLa total lysates.
Date published: 2018-02-14
Rated 3 out of 5 by from Nice antibody We used this antibody for IP last month. It works well for some primary proteins. Overall a decent buy.
Date published: 2017-11-13
Rated 4 out of 5 by from works well I have tried this antibody in cells deprived for glucose. The results were good and clean
Date published: 2017-10-27
Rated 5 out of 5 by from Great results The results are quit stable. Exposure a little bit longer, you will get very clear ban.
Date published: 2017-10-03
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