p-Ribosomal Protein S6 Antibody (50.Ser 235/236)recommended for detection of Ser 235 and Ser 236 phosphorylated Ribosomal Protein S6 of mouse, rat and human origin by WB, IP and ELISA

p-Ribosomal Protein S6 Antibody (50.Ser 235/236): sc-293144

p-Ribosomal Protein S6 Antibody (50.Ser 235/236) is rated 4.8 out of 5 by 4.
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Datasheets

    • p-Ribosomal Protein S6 Antibody (50.Ser 235/236) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Ser 235 and Ser 236 of Ribosomal Protein S6 of human origin
    • recommended for detection of Ser 235 and Ser 236 phosphorylated Ribosomal Protein S6 of mouse, rat and human origin by WB, IP and ELISA
    • available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Ribosomal Protein S6 (50.Ser 235/236): sc-293144.
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What is the recommended starting dilution for Western blot for sc-293144: p-Ribosomal Protein S6 (50.Ser 235/236) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Excelent result The bands were at the expected size and they were nicely visible. Worked great for human fibroblast samples when probing at 1:1000 via WB. Highly recommend!
Date published: 2018-11-08
Rated 5 out of 5 by from Excellent! This antibody is recommended for detection of Ser 235 and Ser 236 phosphorylated Ribosomal Protein S6 of mouse, rat and human origin by WB, IP and ELISA
Date published: 2017-02-02
Rated 5 out of 5 by from WB on Human cancer cell lysates: Very good WB on Human cancer cell lysates: Very good antibody giving publishable data
Date published: 2016-08-29
Rated 4 out of 5 by from Worked well with untreated Worked well with untreated , EGF treated and EGF and lambda protein phosphatase treated HEK293 whole cell lysates by WB with some background. -SCBT QC
Date published: 2014-01-18
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